@inbook{c499f85f7f0342d0a6a6f31697ebf9d0,
title = "Isolation of tumor-infiltrating lymphocytes by ficoll-paque density gradient centrifugation",
abstract = "With the renewed enthusiasm in immuno-oncology, characterization of the tumor immune microenvironment constitutes an essential and unique aspect to the assessment of therapeutics. The isolation of tumor-infiltrating lymphocytes (TILs) is a desirable approach toward the understanding of antitumor immune response. This chapter provides an effective protocol to mechanically dissociate tumor tissue and generate single-cell suspension from excised tumors. TILs are then isolated by Ficoll-Paque density gradient centrifugation. This protocol is applicable to both human and experimental tumors in immunocompetent murine models.",
keywords = "Immune escape, Immunotherapy, Mechanical dissociation, Tumor microenvironment, Tumor-infiltrating lymphocytes",
author = "Tan, \{Yee Sun\} and Lei, \{Yu L.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9167-9\_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "93--99",
booktitle = "Methods in Molecular Biology",
}