Lack of normal CFU-C/suppressor activity in population of alloantigen-stimulated T cells from patients with chronic myelogenous leukemia

T. Maekawa, Y. Sonoda, S. Yokota, T. Abe

Research output: Contribution to journalArticle

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Abstract

Alloantigen-stimulated T cells from patients with Ph1-positive chronic myelogenous leukemia (CML) and normal individuals were obtained by one-way mixed lymphocyte cultures (MLC). Effects of alloantigen-stimulated T cells and MLC supernatants on colony formation were studied in vitro. Alloantigen-stimulated T cells were cocultured with normal allogeneic bone marrow cells (target cells) grown for CFU-C and BFU-E formation. Target cells were also cultured with MLC supernatants. Alloantigen-stimulated T cells from normal controls significantly suppressed CFU-C formation in the presence of human placental conditioned medium (HPCM), while those from CML patients failed to reduce it. MLC supernatants from CML patients were significantly less suppressive on CFU-C formation than those from normal controls. In contrast, not only normal and CML alloantigen-stimulated T cells but also their MLC supernatants increased BFU-E formation in a similar manner. Furthermore, MLC supernatants from normal controls as well as CML patients stimulated the growth of CFU-C from target cells in the absence of HPCM. These results suggest that CML T cells lack the ability to release soluble inhibitors of granulopoiesis, although the production of colony-stimulating factors and burst-promoting activities was equivalent to that of normal T cells after alloantigen stimulation in vitro. The failure of CML T cells to suppress granulopoiesis may contribute to the preferential proliferation of granulocytes in the pathophysiology of CML.

Original languageEnglish (US)
Pages (from-to)316-323
Number of pages8
JournalExperimental Hematology
Volume14
Issue number4
StatePublished - Jan 1 1986

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Isoantigens
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
T-Lymphocytes
Lymphocytes
Population
Erythroid Precursor Cells
Conditioned Culture Medium
Colony-Stimulating Factors
Granulocytes
Bone Marrow Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

Lack of normal CFU-C/suppressor activity in population of alloantigen-stimulated T cells from patients with chronic myelogenous leukemia. / Maekawa, T.; Sonoda, Y.; Yokota, S.; Abe, T.

In: Experimental Hematology, Vol. 14, No. 4, 01.01.1986, p. 316-323.

Research output: Contribution to journalArticle

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