@inproceedings{f70219aa9884493dae79b1f3cc1f6731,
title = "Large scale generation of genetically modified T-cells using micro-electroporators for cancer treatments",
abstract = "To address B-lineage acute lymphoblastic leukemia (B-ALL), we use a bioengineering approach to achieve high-efficient non-viral gene transfer of tumor-specific CAR+ T cells for on-demand use. Specifically, we have developed CD19-specific chimeric antigen receptors (CARs) to redirect the specificity of T cells for B-cell malignancies. We introduce CARs via a non-viral gene transfer: electroporation. We have fabricated and tested a series of high throughput micro-electroporation devices (HitMeDs) with the end result that a HitMeD can electroporate a large number (>109) human T cells within short time period (<4 hours). We connect the HitMeDs to a BTX pulse generator and a monitoring system. We have adapted HitMeDs to the electro-transfer of CAR mRNA which avoids the potential genotoxicity associated with vector integration. After electroporation, 70% to 80% of the T cells express CD19 -specific CAR.",
keywords = "CD19-specific CAR, Cancer, Electroporation, High throughput, mRNA",
author = "Yoonsu Choi and Sourindra Maiti and Carrie Yuen and Helen Huls and Robert Raphael and Killian, {Thomas C.} and Stark, {Daniel J.} and Sorin Lupascu and Lee, {Dean A.} and Shpall, {Elizabeth J.} and Partow Kebriaei and Champlin, {Richard E.} and Biswal, {Sibani L.} and Cooper, {Laurence J.N.}",
year = "2009",
doi = "10.1109/SENSOR.2009.5285590",
language = "English (US)",
isbn = "9781424441938",
series = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
pages = "2226--2229",
booktitle = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
note = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 21-06-2009 Through 25-06-2009",
}