LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review

Kensuke Yamamura; Keisuke Kosumi; Yoshifumi Baba; Kazuto Harada; Feng Gao; Xiaobo Zhang; Lei Zhou; Yuki Kitano; Kota Arima; Takayoshi Kaida; Hideaki Takeyama; Takaaki Higashi; Katsunori Imai; Daisuke Hashimoto; Akira Chikamoto; Xiaodong Tan; Hideo Baba

doi:10.1007/s00595-017-1539-1

LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review

Kensuke Yamamura, Keisuke Kosumi, Yoshifumi Baba, Kazuto Harada, Feng Gao, Xiaobo Zhang, Lei Zhou, Yuki Kitano, Kota Arima, Takayoshi Kaida, Hideaki Takeyama, Takaaki Higashi, Katsunori Imai, Daisuke Hashimoto, Akira Chikamoto, Xiaodong Tan, Hideo Baba

Cancer Prevention Training Program

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Purpose: Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear. Methods: We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level. Results: LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues (p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3–92.8 (n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively). Conclusion: The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.

Original language	English (US)
Pages (from-to)	1450-1459
Number of pages	10
Journal	Surgery Today
Volume	47
Issue number	12
DOIs	https://doi.org/10.1007/s00595-017-1539-1
State	Published - Dec 1 2017

Keywords

Epigenetics
LINE-1
Methylation
Pancreatic cancer
Prognosis

ASJC Scopus subject areas

Surgery

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10.1007/s00595-017-1539-1

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Yamamura, K., Kosumi, K., Baba, Y., Harada, K., Gao, F., Zhang, X., Zhou, L., Kitano, Y., Arima, K., Kaida, T., Takeyama, H., Higashi, T., Imai, K., Hashimoto, D., Chikamoto, A., Tan, X., & Baba, H. (2017). LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review. Surgery Today, 47(12), 1450-1459. https://doi.org/10.1007/s00595-017-1539-1

Yamamura, K, Kosumi, K, Baba, Y, Harada, K, Gao, F, Zhang, X, Zhou, L, Kitano, Y, Arima, K, Kaida, T, Takeyama, H, Higashi, T, Imai, K, Hashimoto, D, Chikamoto, A, Tan, X & Baba, H 2017, 'LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review', Surgery Today, vol. 47, no. 12, pp. 1450-1459. https://doi.org/10.1007/s00595-017-1539-1

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title = "LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review",

abstract = "Purpose: Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear. Methods: We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level. Results: LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues (p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3–92.8 (n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively). Conclusion: The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.",

keywords = "Epigenetics, LINE-1, Methylation, Pancreatic cancer, Prognosis",

author = "Kensuke Yamamura and Keisuke Kosumi and Yoshifumi Baba and Kazuto Harada and Feng Gao and Xiaobo Zhang and Lei Zhou and Yuki Kitano and Kota Arima and Takayoshi Kaida and Hideaki Takeyama and Takaaki Higashi and Katsunori Imai and Daisuke Hashimoto and Akira Chikamoto and Xiaodong Tan and Hideo Baba",

note = "Publisher Copyright: {\textcopyright} 2017, Springer Japan.",

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doi = "10.1007/s00595-017-1539-1",

language = "English (US)",

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TY - JOUR

T1 - LINE-1 methylation level and prognosis in pancreas cancer

T2 - pyrosequencing technology and literature review

AU - Yamamura, Kensuke

AU - Kosumi, Keisuke

AU - Baba, Yoshifumi

AU - Harada, Kazuto

AU - Gao, Feng

AU - Zhang, Xiaobo

AU - Zhou, Lei

AU - Kitano, Yuki

AU - Arima, Kota

AU - Kaida, Takayoshi

AU - Takeyama, Hideaki

AU - Higashi, Takaaki

AU - Imai, Katsunori

AU - Hashimoto, Daisuke

AU - Chikamoto, Akira

AU - Tan, Xiaodong

AU - Baba, Hideo

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Purpose: Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear. Methods: We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level. Results: LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues (p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3–92.8 (n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively). Conclusion: The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.

AB - Purpose: Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear. Methods: We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level. Results: LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues (p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3–92.8 (n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively). Conclusion: The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.

KW - Epigenetics

KW - LINE-1

KW - Methylation

KW - Pancreatic cancer

KW - Prognosis

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U2 - 10.1007/s00595-017-1539-1

DO - 10.1007/s00595-017-1539-1

M3 - Article

C2 - 28536860

AN - SCOPUS:85019851296

SN - 0941-1291

VL - 47

SP - 1450

EP - 1459

JO - Surgery Today

JF - Surgery Today

IS - 12

ER -

LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review

Abstract

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