TY - CHAP
T1 - Liposomal siRNA for ovarian cancer
AU - Mangala, Lingegowda S.
AU - Han, Hee Dong
AU - Lopez-Berestein, Gabriel
AU - Sood, Anil K.
PY - 2009
Y1 - 2009
N2 - Discovery of RNA interference (RNAi) has been one of the most important findings in the last ten years. In recent years, small interfering RNA (siRNA)-mediated gene silencing is beginning to show substantial promise as a new treatment modality in preclinical studies because of its robust gene selective silencing. However, until recently, delivery of siRNA in vivo was a major impediment to its use as a therapeutic modality. We have used a neutral liposome, 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC), for highly efficient in vivo siRNA delivery. Using siRNA tagged with Alexa-555, incorporated in DOPC liposomes, we have demonstrated efficient intra-tumoral delivery following either intraperitoneal or intravenous injection. Furthermore, EphA2-targeted siRNA in DOPC liposomes showed significant target modulation and anti-tumor efficacy.
AB - Discovery of RNA interference (RNAi) has been one of the most important findings in the last ten years. In recent years, small interfering RNA (siRNA)-mediated gene silencing is beginning to show substantial promise as a new treatment modality in preclinical studies because of its robust gene selective silencing. However, until recently, delivery of siRNA in vivo was a major impediment to its use as a therapeutic modality. We have used a neutral liposome, 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC), for highly efficient in vivo siRNA delivery. Using siRNA tagged with Alexa-555, incorporated in DOPC liposomes, we have demonstrated efficient intra-tumoral delivery following either intraperitoneal or intravenous injection. Furthermore, EphA2-targeted siRNA in DOPC liposomes showed significant target modulation and anti-tumor efficacy.
KW - DOPC
KW - EphA2
KW - RNA interference
KW - neutral liposome
KW - ovarian carcinoma
KW - siRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=70349330919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349330919&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-295-7_3
DO - 10.1007/978-1-60327-295-7_3
M3 - Chapter
C2 - 19495686
AN - SCOPUS:70349330919
SN - 9781603272940
T3 - Methods in Molecular Biology
SP - 29
EP - 42
BT - Therapeutic Applications of RNAi
A2 - Reidhaar-Olson, John
A2 - Rondinone, Cristina
ER -