lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators

Alena Blank-Giwojna, Anna Postepska-Igielska, Ingrid Grummt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Blank-Giwojna et al. demonstrate that the antisense RNA KHPS1 forms an RNA-DNA triplex at the SPHK1 enhancer. Tethering KHPS1 to the enhancer is required for recruitment of E2F1 and p300, transcription of enhancer-derived RNA, and activation of SPHK1 expression. The results uncover a triplex-driven feedforward mechanism of transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)2904-2915.e4
JournalCell Reports
Volume26
Issue number11
DOIs
StatePublished - Mar 12 2019

Fingerprint

Long Noncoding RNA
Epigenomics
RNA
Antisense RNA
Transcription
Chemical activation
triplex DNA

Keywords

  • CTCF
  • E2F1
  • RNA-DNA triplexes
  • SPHK1
  • eRNA
  • eRNA
  • lncRNA
  • p300
  • poised enhancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators. / Blank-Giwojna, Alena; Postepska-Igielska, Anna; Grummt, Ingrid.

In: Cell Reports, Vol. 26, No. 11, 12.03.2019, p. 2904-2915.e4.

Research output: Contribution to journalArticle

Blank-Giwojna, Alena ; Postepska-Igielska, Anna ; Grummt, Ingrid. / lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators. In: Cell Reports. 2019 ; Vol. 26, No. 11. pp. 2904-2915.e4.
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