Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes

Jörge J. Yunis; Macus Tien Kuo; Grady F. Saunders

doi:10.1007/BF00288617

Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes

Jörge J. Yunis, Macus Tien Kuo, Grady F. Saunders

Translational Molecular Pathology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil₂ by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.

Original language	English (US)
Pages (from-to)	335-344
Number of pages	10
Journal	Chromosoma
Volume	61
Issue number	4
DOIs	https://doi.org/10.1007/BF00288617
State	Published - Dec 1977

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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10.1007/BF00288617

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title = "Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes",

abstract = "Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.",

author = "Yunis, {J{\"o}rge J.} and Kuo, {Macus Tien} and Saunders, {Grady F.}",

year = "1977",

month = dec,

doi = "10.1007/BF00288617",

language = "English (US)",

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AU - Yunis, Jörge J.

AU - Kuo, Macus Tien

AU - Saunders, Grady F.

PY - 1977/12

Y1 - 1977/12

N2 - Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.

AB - Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.

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Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes

Abstract

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