mdm-2 Inhibits the G1 arrest and apoptosis functions of the p53 tumor suppressor protein

Jiandong Chen, Xiangwei Wu, Jiayuh Lin, Arnold J. Levine

Research output: Contribution to journalArticle

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Abstract

The mdm-2 gene encodes a 90-kDa polypeptide that binds specifically to the p53 tumor suppressor protein. This physical interaction results in the inhibition of the transcriptional functions of p53 (J. Chen, J. Lin, and A. J. Levine, Mol. Med. 1:142-152, 1995, and J. Momand, G. P. Zambetti, D. C. Olson, D. George, and A. J. Levine, Cell 69:1237-1245, 1992). Experiments are described that demonstrate the ability of mdm-2 to abrogate both the p53- mediated cell cycle arrest and the apoptosis functions. In addition, the results presented here suggest that mdm-2 binding to p53 and the resultant inhibition of p53 transcription functions are critical for reversing p53- mediated cell cycle arrest. The N-terminal half or domain of the mdm-2 protein is sufficient to regulate these biological activities of p53, consistent with the possibility that the highly conserved central acidic region and the C-terminal putative zinc fingers of mdm-2 may encode other functions.

Original languageEnglish (US)
Pages (from-to)2445-2452
Number of pages8
JournalMolecular and cellular biology
Volume16
Issue number5
DOIs
StatePublished - May 1996

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Tumor Suppressor Protein p53
Cell Cycle Checkpoints
Proto-Oncogene Proteins c-mdm2
Apoptosis
Zinc Fingers
Peptides
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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mdm-2 Inhibits the G1 arrest and apoptosis functions of the p53 tumor suppressor protein. / Chen, Jiandong; Wu, Xiangwei; Lin, Jiayuh; Levine, Arnold J.

In: Molecular and cellular biology, Vol. 16, No. 5, 05.1996, p. 2445-2452.

Research output: Contribution to journalArticle

Chen, Jiandong ; Wu, Xiangwei ; Lin, Jiayuh ; Levine, Arnold J. / mdm-2 Inhibits the G1 arrest and apoptosis functions of the p53 tumor suppressor protein. In: Molecular and cellular biology. 1996 ; Vol. 16, No. 5. pp. 2445-2452.
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