TY - JOUR
T1 - Metastasis
T2 - Quantitative analysis of distribution and fate of tumor emboli labeled with 125l-5-lodo-2'-deoxyuridine
AU - Fidler, Isaiah J.
N1 - Funding Information:
1 Received April 27, 1970; accepted June 24,1970. 2 This investigation was performed in partial fulfillment of the requirements for the degree of Ph.D. at the University of Pennsylvania. 3 Supported by Public Health Service research grant CA 05022 from the National Cancer Institute. • I thank Dr. Irving Zeidman for his valuable advice and support. 5 Present address: Department of Pathology, School of Dental Medicine, University of Pennsylvania.
PY - 1970/10
Y1 - 1970/10
N2 - 5-iodo-2'-deoxyuridine was used to label pure B16 melanoma cells grown in tissue culture. In vitro and in vivo studies established the optimal, nontoxic labeling dose. C57BL/6J mice were given intravenous injections of labeled cells and killed at various intervals after injection. Blood and organs were collected, processed, and monitored. The label was released only after cell death. It was poorly reutilized and quickly eliminated. Hence, organ radioactivity represented almost exclusively the number of live cells present when the animals were killed. The data permitted the following conclusions: The lung contained most of the tumor cells at all intervals. Some tumor cell emboli recirculated. Tumor cells in the lung decreased in numbers at about 5 minutes post injection and returned to high levels by 10 minutes post injection. Tumor cells died rapidly, and about 1 % of cells survived after 24 hours. About 400 melanoma cells were in the lung after 14 days, and those yielded an average of 78 metastases. Apparently very few surviving tumor cells are needed to establish a metastasis.
AB - 5-iodo-2'-deoxyuridine was used to label pure B16 melanoma cells grown in tissue culture. In vitro and in vivo studies established the optimal, nontoxic labeling dose. C57BL/6J mice were given intravenous injections of labeled cells and killed at various intervals after injection. Blood and organs were collected, processed, and monitored. The label was released only after cell death. It was poorly reutilized and quickly eliminated. Hence, organ radioactivity represented almost exclusively the number of live cells present when the animals were killed. The data permitted the following conclusions: The lung contained most of the tumor cells at all intervals. Some tumor cell emboli recirculated. Tumor cells in the lung decreased in numbers at about 5 minutes post injection and returned to high levels by 10 minutes post injection. Tumor cells died rapidly, and about 1 % of cells survived after 24 hours. About 400 melanoma cells were in the lung after 14 days, and those yielded an average of 78 metastases. Apparently very few surviving tumor cells are needed to establish a metastasis.
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U2 - 10.1093/jnci/45.4.773
DO - 10.1093/jnci/45.4.773
M3 - Article
C2 - 5513503
AN - SCOPUS:0014865396
SN - 0027-8874
VL - 45
SP - 773
EP - 782
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -