Microtubules: Evidence for 13 protofilaments

Lewis G. Tilney; Joseph Bryan; Doris J. Bush; Keigi Fujiwara; Mark S. Mooseker; Douglas B. Murphy; Daniel H. Snyder

doi:10.1083/jcb.59.2.267

Microtubules: Evidence for 13 protofilaments

Lewis G. Tilney, Joseph Bryan, Doris J. Bush, Keigi Fujiwara, Mark S. Mooseker, Douglas B. Murphy, Daniel H. Snyder

Cardiology

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337 Scopus citations

Abstract

When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed : those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.

Original language	English (US)
Pages (from-to)	267-275
Number of pages	9
Journal	Journal of Cell Biology
Volume	59
Issue number	2
DOIs	https://doi.org/10.1083/jcb.59.2.267
State	Published - Nov 1 1973

ASJC Scopus subject areas

Cell Biology

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abstract = "When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed : those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.",

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AU - Tilney, Lewis G.

AU - Bryan, Joseph

AU - Bush, Doris J.

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AU - Mooseker, Mark S.

AU - Murphy, Douglas B.

AU - Snyder, Daniel H.

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AB - When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed : those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.

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Microtubules: Evidence for 13 protofilaments

Abstract

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