TY - JOUR
T1 - Molecular cloning and characterization of a novel mouse epidermal differentiation gene and its promoter
AU - Wang, Aijin
AU - Johnson, David G.
AU - MacLeod, Michael C.
N1 - Funding Information:
We thank S. Hensley and A. Tanzillo-Swarts for expert sequencing, J. Smith for her careful preparation of keratinocytes from new-born mouse skin, J. DiGiovanni and S. Carbajal for total RNA from mouse skin papillomas, R. Schneider-Broussard, D. Wang, and R. Rounbehler for total RNA from transgenic mouse epidermis and skin carcinomas, and R. Deen and J. Ing for assistance in manuscript preparation. This work was supported by NIH Grants CA35581 (M.C.M.) and CA79648 (D.G.J.) and Center Grants ES07784 and CA16672.
PY - 2001/5/1
Y1 - 2001/5/1
N2 - The transcription factor E2F1 is an important regulator of cell proliferation, apoptosis, and differentiation. A novel mouse gene (Eig3) was originally identified as up-regulated in E2F1-overexpressing keratinocytes by the rapid analysis of gene expression technique. An apparently full-length cDNA and a 2.8-kb genomic fragment containing the entire gene have been cloned. Sequence comparisons suggest that Eig3 is homologous to a human epidermal differentiation gene, XP5, and belongs to a family of at least 10 murine genes that are related to the small proline-rich genes involved in skin differentiation. Eig3 was expressed in adult mouse stomach and epidermis and overexpressed in keratinocytes transgenic for E2F1 or E2F4, but not in c-myc transgenics. Interestingly, Eig3 expression was highly increased in mouse skin papillomas but not in squamous carcinomas. Since there is no E2F consensus binding sequence in the promoter or first intron of Eig3, E2F regulation may be indirect.
AB - The transcription factor E2F1 is an important regulator of cell proliferation, apoptosis, and differentiation. A novel mouse gene (Eig3) was originally identified as up-regulated in E2F1-overexpressing keratinocytes by the rapid analysis of gene expression technique. An apparently full-length cDNA and a 2.8-kb genomic fragment containing the entire gene have been cloned. Sequence comparisons suggest that Eig3 is homologous to a human epidermal differentiation gene, XP5, and belongs to a family of at least 10 murine genes that are related to the small proline-rich genes involved in skin differentiation. Eig3 was expressed in adult mouse stomach and epidermis and overexpressed in keratinocytes transgenic for E2F1 or E2F4, but not in c-myc transgenics. Interestingly, Eig3 expression was highly increased in mouse skin papillomas but not in squamous carcinomas. Since there is no E2F consensus binding sequence in the promoter or first intron of Eig3, E2F regulation may be indirect.
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U2 - 10.1006/geno.2001.6518
DO - 10.1006/geno.2001.6518
M3 - Article
C2 - 11350120
AN - SCOPUS:0035336278
SN - 0888-7543
VL - 73
SP - 284
EP - 290
JO - Genomics
JF - Genomics
IS - 3
ER -