Abstract
There is strong evidence that multistep tumorigenesis begins with the acquisition of somatic mutations which promote genomic instability. Genomic instability is an important malignant trait because genomic instability can generate the genetic diversity that is necessary for the transforming cell to acquire increasingly variable and aggressive tumor phenotypes. Genomic instability often manifests in the form of chromosomal instability (CIN) leading to the induction of aneuploidy, a phenomenon identified by high resolution molecular cytogenetic techniques. Fluorescent in situ hybridization (FISH) and Array Comparative Genomic Hybridization (aCGH) are two high resolution molecular cytogenetic techniques that allow detection of chromosomal aneuploidy and structural rearrangements occurring in pre-malignant and malignant lesions during tumor progression and invasion. These high resolution molecular cytogenetic techniques are used for genetic screening of single cells in pre-malignant and precursor malignant lesions as well as in exfoliated cells from body fluids and excreta. Consequently, molecular cytogenetic testing offers the promise of an extremely powerful method of risk assessment and early detection of cancer.
Original language | English (US) |
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Title of host publication | Translational Pathology of Early Cancer |
Publisher | IOS Press |
Pages | 113-132 |
Number of pages | 20 |
ISBN (Print) | 9781614990239 |
DOIs | |
State | Published - Mar 2012 |
Keywords
- Aneuploidy
- Array Comparative Genomic Hybridization(aCGH)
- Chromosomal instability (CIN)
- Chromosomal rearrangements
- Chromosome deletion
- Fluorescence in situ hybridization (FISH)
- Gene amplification
- Gene fusion
- Genetic mutation
- Microenvironment
- Spindle assembly checkpoint
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Medicine