Mouse apolipoprotein B editing complex 3 (APOBEC3) is expressed in germ cells and interacts with dead-end (DND1)

Chitralekha Bhattacharya, Sita Aggarwal, Madhu Kumar, Amatul Ali, Angabin Matin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The dead-end (Dnd1) gene is essential for maintaining the viability of germ cells. Inactivation of Dnd1 results in sterility and testicular tumors. The Dnd1 encoded protein, DND1, is able to bind to the 3′-untranslated region (UTR) of messenger RNAs (mRNAs) to displace micro-RNA (miRNA) interaction with mRNA. Thus, one function of DND1 is to prevent miRNA mediated repression of mRNA. We report that DND1 interacts specifically with APOBEC3. APOBEC3 is a multifunctional protein. It inhibits retroviral replication. In addition, recent studies show that APOBEC3 interacts with cellular RNA-binding proteins and to mRNA to inhibit miRNA-mediated repression of mRNA. Methodology/Principal Findings: Here we show that DND1 specifically interacts with another cellular protein, APOBEC3. We present our data which shows that DND1 co-immunoprecipitates APOBEC3 from mammalian cells and also endogenous APOBEC3 from mouse gonads. Whether the two proteins interact directly remains to be elucidated. We show that both DND1 and APOBEC3 are expressed in germ cells and in the early gonads of mouse embryo. Expression of fluorescentlytagged DND1 and APOBEC3 indicate they localize to the cytoplasm and when DND1 and APOBEC3 are expressed together in cells, they sequester near peri-nuclear sites. Conclusions/Significance:The 3′-UTR of mRNAs generally encode multiple miRNA binding sites as well as binding sites for a variety of RNA binding proteins. In light of our findings of DND1-APOBEC3 interaction and taking into consideration reports that DND1 and APOBEC3 bind to mRNA to inhibit miRNA mediated repression, our studies implicate a possible role of DND1-APOBEC3 interaction in modulating miRNA-mediated mRNA repression. The interaction of DND1 and APOBEC3 could be one mechanism for maintaining viability of germ cells and for preventing germ cell tumor development.

Original languageEnglish (US)
Article numbere2315
JournalPloS one
Volume3
Issue number5
DOIs
StatePublished - May 28 2008

Fingerprint

apolipoprotein B
Apolipoproteins B
Germ Cells
germ cells
Cells
messenger RNA
mice
MicroRNAs
microRNA
Messenger RNA
RNA-binding proteins
RNA-Binding Proteins
Gonads
3' Untranslated Regions
3' untranslated regions
Mouse Apob protein
gonads
Tumors
binding sites
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Mouse apolipoprotein B editing complex 3 (APOBEC3) is expressed in germ cells and interacts with dead-end (DND1). / Bhattacharya, Chitralekha; Aggarwal, Sita; Kumar, Madhu; Ali, Amatul; Matin, Angabin.

In: PloS one, Vol. 3, No. 5, e2315, 28.05.2008.

Research output: Contribution to journalArticle

Bhattacharya, Chitralekha ; Aggarwal, Sita ; Kumar, Madhu ; Ali, Amatul ; Matin, Angabin. / Mouse apolipoprotein B editing complex 3 (APOBEC3) is expressed in germ cells and interacts with dead-end (DND1). In: PloS one. 2008 ; Vol. 3, No. 5.
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