TY - JOUR
T1 - Mouse model for pre-clinical study of human cancer immunotherapy
AU - Ya, Zhiya
AU - Hailemichael, Yared
AU - Overwijk, Willem
AU - Restifo, Nicholas P.
N1 - Publisher Copyright:
© 2015 by John Wiley & Sons, Inc.
PY - 2015
Y1 - 2015
N2 - This unit describes protocols for developing tumors in mice, including subcutaneous growth, pulmonary metastases of B16 melanoma, and spontaneous melanoma in B-Raf V600E/PTEN deletion transgenic mouse models. Two immunization methods to prevent B16 tumor growth are described usingB16.GMCSF and recombinant vaccinia virus. A therapeutic approach is also included that uses adoptive transfer of tumor antigen-specific T cells. Methods including CTL induction, isolation, testing, and genetic modification of mouse T cells for adoptive transfer by using retrovirus-expressing genes of interest are provided. Additional sections, including growing B16 melanoma, enumerating pulmonary metastases, tumor imaging technique, and use of recombinant viruses for vaccination, are discussed together with safety concerns.
AB - This unit describes protocols for developing tumors in mice, including subcutaneous growth, pulmonary metastases of B16 melanoma, and spontaneous melanoma in B-Raf V600E/PTEN deletion transgenic mouse models. Two immunization methods to prevent B16 tumor growth are described usingB16.GMCSF and recombinant vaccinia virus. A therapeutic approach is also included that uses adoptive transfer of tumor antigen-specific T cells. Methods including CTL induction, isolation, testing, and genetic modification of mouse T cells for adoptive transfer by using retrovirus-expressing genes of interest are provided. Additional sections, including growing B16 melanoma, enumerating pulmonary metastases, tumor imaging technique, and use of recombinant viruses for vaccination, are discussed together with safety concerns.
KW - B16 melanoma
KW - Translational cancer immunotherapy
KW - Tumor imaging
KW - Use of recombinant viruses for vaccination
UR - http://www.scopus.com/inward/record.url?scp=84949225193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949225193&partnerID=8YFLogxK
U2 - 10.1002/0471142735.im2001s108
DO - 10.1002/0471142735.im2001s108
M3 - Article
C2 - 25640991
AN - SCOPUS:84949225193
SN - 1934-3671
VL - 2015
SP - 20.1.1-20.1.43
JO - Current Protocols in Immunology
JF - Current Protocols in Immunology
ER -