Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films

Xinjian Liu; Xiaofeng Zheng; Yi Xu; Genxi Li

doi:10.1016/j.molcatb.2005.01.002

Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films

Xinjian Liu
, Xiaofeng Zheng
, Yi Xu
, Genxi Li

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

By entrapping cytochrome c (Cyt c) in phosphatidylcholine (PC) film, we have obtained the direct electrochemistry of the protein. Meanwhile, the catalytic reduction of nitric oxide (NO) has been investigated. Besides the pair of peaks corresponding to the redox reactions of Cyt c, two new cathodic peaks can be observed after the addition of NO into the test solution. One, located at -0.510 V, is proposed to come from the formation of hydroxylamine. The other, at -0.690 V, is assigned to the electrochemical reduction of NO. These observations are very different from the previous reports, because only one peak can be obtained for the other studies. The difference is related to the electron transfer rate and the escape rate of the products in the NO reduction process. This study might bring clearer insight into the reduction mechanism of NO.

Original language	English (US)
Pages (from-to)	9-13
Number of pages	5
Journal	Journal of Molecular Catalysis B: Enzymatic
Volume	33
Issue number	1-2
DOIs	https://doi.org/10.1016/j.molcatb.2005.01.002
State	Published - May 3 2005
Externally published	Yes

Keywords

Catalytic reduction
Cytochrome c
Electrochemistry
Nitric oxide
Phosphatidylcholine

ASJC Scopus subject areas

Catalysis
Bioengineering
Biochemistry
Process Chemistry and Technology

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10.1016/j.molcatb.2005.01.002

Cite this

@article{61e67a6843544b449838cde61159f4cc,

title = "Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films",

abstract = "By entrapping cytochrome c (Cyt c) in phosphatidylcholine (PC) film, we have obtained the direct electrochemistry of the protein. Meanwhile, the catalytic reduction of nitric oxide (NO) has been investigated. Besides the pair of peaks corresponding to the redox reactions of Cyt c, two new cathodic peaks can be observed after the addition of NO into the test solution. One, located at -0.510 V, is proposed to come from the formation of hydroxylamine. The other, at -0.690 V, is assigned to the electrochemical reduction of NO. These observations are very different from the previous reports, because only one peak can be obtained for the other studies. The difference is related to the electron transfer rate and the escape rate of the products in the NO reduction process. This study might bring clearer insight into the reduction mechanism of NO.",

keywords = "Catalytic reduction, Cytochrome c, Electrochemistry, Nitric oxide, Phosphatidylcholine",

author = "Xinjian Liu and Xiaofeng Zheng and Yi Xu and Genxi Li",

note = "Funding Information: We appreciate the support of the National Natural Science Foundation (grant no. 30070214) and the Ministry of Education (grant no. 01087), PR China, for this research.",

year = "2005",

month = may,

day = "3",

doi = "10.1016/j.molcatb.2005.01.002",

language = "English (US)",

volume = "33",

pages = "9--13",

journal = "Journal of Molecular Catalysis B: Enzymatic",

issn = "1381-1177",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films

AU - Liu, Xinjian

AU - Zheng, Xiaofeng

AU - Xu, Yi

AU - Li, Genxi

N1 - Funding Information: We appreciate the support of the National Natural Science Foundation (grant no. 30070214) and the Ministry of Education (grant no. 01087), PR China, for this research.

PY - 2005/5/3

Y1 - 2005/5/3

N2 - By entrapping cytochrome c (Cyt c) in phosphatidylcholine (PC) film, we have obtained the direct electrochemistry of the protein. Meanwhile, the catalytic reduction of nitric oxide (NO) has been investigated. Besides the pair of peaks corresponding to the redox reactions of Cyt c, two new cathodic peaks can be observed after the addition of NO into the test solution. One, located at -0.510 V, is proposed to come from the formation of hydroxylamine. The other, at -0.690 V, is assigned to the electrochemical reduction of NO. These observations are very different from the previous reports, because only one peak can be obtained for the other studies. The difference is related to the electron transfer rate and the escape rate of the products in the NO reduction process. This study might bring clearer insight into the reduction mechanism of NO.

AB - By entrapping cytochrome c (Cyt c) in phosphatidylcholine (PC) film, we have obtained the direct electrochemistry of the protein. Meanwhile, the catalytic reduction of nitric oxide (NO) has been investigated. Besides the pair of peaks corresponding to the redox reactions of Cyt c, two new cathodic peaks can be observed after the addition of NO into the test solution. One, located at -0.510 V, is proposed to come from the formation of hydroxylamine. The other, at -0.690 V, is assigned to the electrochemical reduction of NO. These observations are very different from the previous reports, because only one peak can be obtained for the other studies. The difference is related to the electron transfer rate and the escape rate of the products in the NO reduction process. This study might bring clearer insight into the reduction mechanism of NO.

KW - Catalytic reduction

KW - Cytochrome c

KW - Electrochemistry

KW - Nitric oxide

KW - Phosphatidylcholine

UR - https://www.scopus.com/pages/publications/17844391517

UR - https://www.scopus.com/pages/publications/17844391517#tab=citedBy

U2 - 10.1016/j.molcatb.2005.01.002

DO - 10.1016/j.molcatb.2005.01.002

M3 - Article

AN - SCOPUS:17844391517

SN - 1381-1177

VL - 33

SP - 9

EP - 13

JO - Journal of Molecular Catalysis B: Enzymatic

JF - Journal of Molecular Catalysis B: Enzymatic

IS - 1-2

ER -

Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films

Abstract

Keywords

ASJC Scopus subject areas

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