Multiplex Immunofluorescence Assays

Research output: Contribution to journalArticle

Abstract

Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue section emerged in the last years as very powerful tools to study tumor immune contexture. These revolutionary technologies are providing a deep methodology for tumor evaluation in formalin-fixed and paraffin-embedded (FFPE) to improve the understanding of tumor microenvironment, new targets for treatment, prognostic and predictive biomarkers, and translational studies. Multiplexed imaging platforms allow for the identification of several antigens simultaneously from a single tissue section, core needle biopsies, and tissue microarrays. In recent years, multiplexed imaging has improved the abilities to characterize the different types of cell populations in malignant and non-malignant tissues, and their spatial distribution in relationship to clinical outcomes. Multiplexed technologies associated with digital image analysis software offer a high-quality throughput assay to study cancer specimens at multiple time points before, during and after treatment. The aim of this chapter is to provide a review of multiplexed imaging covering its fundamentals, advantages, disadvantages, and material and methods for staining applied to FFPE tumor tissues and focusing on the use of multiplex immunofluorescence with tyramine signal amplification staining for immune profiling and translational research.

Original languageEnglish (US)
Pages (from-to)467-495
Number of pages29
JournalMethods in molecular biology (Clifton, N.J.)
Volume2055
DOIs
StatePublished - Jan 1 2020

Fingerprint

Fluorescent Antibody Technique
Paraffin
Formaldehyde
Neoplasms
Staining and Labeling
Technology
Large-Core Needle Biopsy
Tyramine
Translational Medical Research
Tumor Microenvironment
Epitopes
Software
Biomarkers
Antigens
Therapeutics
Population

Keywords

  • Cancer tissue
  • Digital image analysis
  • Immune-profiling
  • Tyramide signal amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Multiplex Immunofluorescence Assays. / Francisco-Cruz, Alejandro; Parra Cuentas, Edwin Roger; Tetzlaff, Michael; Wistuba, Ignacio Ivan.

In: Methods in molecular biology (Clifton, N.J.), Vol. 2055, 01.01.2020, p. 467-495.

Research output: Contribution to journalArticle

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