Mutant p53 in concert with an interleukin-27 receptor alpha deficiency causes spontaneous liver inflammation, fibrosis, and steatosis in mice

Denada Dibra, Xueqing Xia, Abhisek Mitra, Jeffry J. Cutrera, Guillermina Lozano, Shulin Li

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The cellular and molecular etiology of unresolved chronic liver inflammation remains obscure. Whereas mutant p53 has gain-of-function properties in tumors, the role of this protein in liver inflammation is unknown. Herein, mutant p53R172H is mechanistically linked to spontaneous and sustained liver inflammation and steatosis when combined with the absence of interleukin-27 (IL27) signaling (IL27RA), resembling the phenotype observed in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) patients. Indeed, these mice develop, with age, hepatocyte necrosis, immune cell infiltration, fibrosis, and micro- and macrosteatosis; however, these phenotypes are absent in mutant p53R172H or IL27RA-/- mice. Mechanistically, endothelin A receptor (ETAR)-positive macrophages are highly accumulated in the inflamed liver, and chemical inhibition of ETAR signaling reverses the observed phenotype and negatively regulates mutant p53 levels in macrophages. Conclusion: The combination of mutant p53 and IL27RA-/- causes spontaneous liver inflammation, steatosis, and fibrosis in vivo, whereas either gene alone in vivo has no effects on the liver.

Original languageEnglish (US)
Pages (from-to)1000-1012
Number of pages13
JournalHepatology
Volume63
Issue number3
DOIs
StatePublished - Mar 1 2016

ASJC Scopus subject areas

  • Hepatology

MD Anderson CCSG core facilities

  • Genetically Engineered Mouse Facility
  • Research Animal Support Facility

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