TY - CHAP
T1 - Nanofluidic Strategies for Cancer Research
AU - Wang, Miao
AU - Huang, Po Jung
AU - Kameoka, Jun
AU - Chou, Chao Kai
AU - Tsou, Pei Hsiang
AU - Yamaguchi, Hirohito
AU - Hung, Mien Chie
N1 - Publisher Copyright:
© The Royal Society of Chemistry 2017.
PY - 2017
Y1 - 2017
N2 - Nanofluidic system can be used as powerful tool for detecting single molecules through fluorescence correlation spectroscopy (FCS). Several types of nanofluidic channels, such as hollow nanofibres or nanotrenches, can be constructed on quartz wafers, though electrospinning and nanolithography, respectively. The advantages of nanofluidic channels in molecule detection are not only in reducing the amount of volume of analyte, but also for improving electrokinetic molecule transport. Therefore, small molecules, like proteins or DNA, can be detected in nanochannels. Furthermore, nanofluidic channels can be used to monitor protein-protein, post translational modification, protein-DNA, and protein-RNA interactions, which rely on labelling proteins of interest within fluorescent molecules, in tissue samples directly. Based on these results, nanofluidic channels can be used in diagnostic application for early diagnosis of cancers and drug screenings.
AB - Nanofluidic system can be used as powerful tool for detecting single molecules through fluorescence correlation spectroscopy (FCS). Several types of nanofluidic channels, such as hollow nanofibres or nanotrenches, can be constructed on quartz wafers, though electrospinning and nanolithography, respectively. The advantages of nanofluidic channels in molecule detection are not only in reducing the amount of volume of analyte, but also for improving electrokinetic molecule transport. Therefore, small molecules, like proteins or DNA, can be detected in nanochannels. Furthermore, nanofluidic channels can be used to monitor protein-protein, post translational modification, protein-DNA, and protein-RNA interactions, which rely on labelling proteins of interest within fluorescent molecules, in tissue samples directly. Based on these results, nanofluidic channels can be used in diagnostic application for early diagnosis of cancers and drug screenings.
UR - http://www.scopus.com/inward/record.url?scp=85006713687&partnerID=8YFLogxK
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U2 - 10.1039/9781849735230-00114
DO - 10.1039/9781849735230-00114
M3 - Chapter
AN - SCOPUS:85006713687
T3 - RSC Nanoscience and Nanotechnology
SP - 114
EP - 149
BT - Nanofluidics, 2nd Edition
A2 - O'Brien, Paul
A2 - Edel, Joshua
A2 - Ivanov, Aleksandar
A2 - Kim, MinJun
PB - Royal Society of Chemistry
ER -