Near real time confocal microscopy of amelanotic tissue: Detection of dysplasia in ex-vivo cervical tissue

T. G. Collier, A. Lacy, A. Malpica, M. Follen, R. Richards-Kortum

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations

Abstract

Invasive carcinoma of the uterine cervix is preceded by a number of morphological intraepithelial changes. Real-time confocal microscopy has been demonstrated to be successful in imaging subcellular detail in-vivo. The purpose of this pilot study is to image ex-vivo tissue using a near real time confocal microscope and determine whether the images can be used to distinguish between normal and dysplastic tissue. Biopsies from 19 patients were imaged at various depths with our confocal microscope. Nuclear morphologic features were extracted from the confocal images. The morphologic feature measurements compare well with the pathologic examination. Discriminating the images for the presence of dysplasia using morphologic feature detection resulted in a sensitivity and specificity of 100% and 91%, respectively.

Original languageEnglish (US)
Pages (from-to)979-981
Number of pages3
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Keywords

  • Amelanotic tissue
  • Confocal microscopy
  • Dysplasia

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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