TY - JOUR
T1 - New vaccinia virus promoter as a potential candidate for future vaccines
AU - Di Pilato, Mauro
AU - Mejías-Pérez, Ernesto
AU - Gómez, Carmen Elena
AU - Perdiguero, Beatriz
AU - Sorzano, Carlos Oscar S.
AU - Esteban, Mariano
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/12
Y1 - 2013/12
N2 - Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.
AB - Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.
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U2 - 10.1099/vir.0.057299-0
DO - 10.1099/vir.0.057299-0
M3 - Article
C2 - 24077296
AN - SCOPUS:84887550005
SN - 0022-1317
VL - 94
SP - 2771
EP - 2776
JO - Journal of General Virology
JF - Journal of General Virology
IS - PART 12
ER -