Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei

Siwei Yang, Daniela Köhler, Kathrin Teller, Thomas Cremer, Roland Eils, Karl Rohr

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an intensity-based approach for non-rigid registration to normalize 3D multi-channel microscopy images of different cell nuclei. A main problem with cell nuclei images is that the intensity structure of different nuclei differs very much, and thus an intensitybased scheme cannot be used directly. Instead, we first perform a segmentation of the images, smooth them by a Gaussian filter, and then apply an intensity-based algorithm. To reduce the computation time we use a multi-resolution scheme. Our approach has been successfully applied using 2D cell-like synthetic images, 3D phantom images, as well as 3D multi-channel microscopy images representing different chromosome regions and gene regions (BACs).

Original languageEnglish (US)
Title of host publicationBildverarbeitung fur die Medizin 2006
Subtitle of host publicationAlgorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops
Pages364-368
Number of pages5
StatePublished - Dec 1 2006
EventWorkshops Bildverarbeitung fur die Medizin: Algorithmen Systeme Anwendungen, BVM 2006 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2006 - Hamburg, Germany
Duration: Mar 19 2006Mar 21 2006

Publication series

NameInformatik aktuell
ISSN (Print)1431-472X

Other

OtherWorkshops Bildverarbeitung fur die Medizin: Algorithmen Systeme Anwendungen, BVM 2006 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2006
CountryGermany
CityHamburg
Period3/19/063/21/06

Fingerprint

Non-rigid Registration
Microscopy
Normalization
Nucleus
Microscopic examination
Cells
Cell
Chromosomes
Genes
Gaussian Filter
Normalize
3D Image
Phantom
Multiresolution
Chromosome
Segmentation
Gene

ASJC Scopus subject areas

  • Modeling and Simulation

Cite this

Yang, S., Köhler, D., Teller, K., Cremer, T., Eils, R., & Rohr, K. (2006). Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei. In Bildverarbeitung fur die Medizin 2006: Algorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops (pp. 364-368). (Informatik aktuell).

Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei. / Yang, Siwei; Köhler, Daniela; Teller, Kathrin; Cremer, Thomas; Eils, Roland; Rohr, Karl.

Bildverarbeitung fur die Medizin 2006: Algorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops. 2006. p. 364-368 (Informatik aktuell).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yang, S, Köhler, D, Teller, K, Cremer, T, Eils, R & Rohr, K 2006, Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei. in Bildverarbeitung fur die Medizin 2006: Algorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops. Informatik aktuell, pp. 364-368, Workshops Bildverarbeitung fur die Medizin: Algorithmen Systeme Anwendungen, BVM 2006 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2006, Hamburg, Germany, 3/19/06.
Yang S, Köhler D, Teller K, Cremer T, Eils R, Rohr K. Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei. In Bildverarbeitung fur die Medizin 2006: Algorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops. 2006. p. 364-368. (Informatik aktuell).
Yang, Siwei ; Köhler, Daniela ; Teller, Kathrin ; Cremer, Thomas ; Eils, Roland ; Rohr, Karl. / Non-rigid registration of 3D microscopy images for the normalization of different cell nuclei. Bildverarbeitung fur die Medizin 2006: Algorithmen - Systeme - Anwendungen, BVM 2006 - Proceedings des Workshops. 2006. pp. 364-368 (Informatik aktuell).
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