Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence

Akira Shimuzu, Shigeru Kondo, Shun Ichi Takeda, Junji Yodoi, Norio Ishida, Hisataka Sabe, Hisao Osawa, Tibor Diamantstein, Toshio Nikaido, Tasuku Honjo

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We have cloned cDNA encoding the mouse interleukin-2(IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the antigen recognized by the monoclonal antibody against the murine IL-2 receptor. The cDNA identified 4 species of mRNA (4.5, 3.5, 2.2 and 1.5 kb) of the mouse IL-2 receptor in CTLL cells. Difference in the length of mRNA seems to be ascribed to the variable length of the 3′ untranslated sequence. Total nucleotide sequence (̃1400 bp) of this cDNA was determined and compared with the human receptor. The nucleotide and amino acid sequences of the IL-2 receptor are 70% and 60%, respectively, homologous in average between the two species. The comparison has revealed several conserved regions localized to particular exons such as transmembrane and cytoplasmic portions, suggesting that these regions are important for receptor function and its regulation.

Original languageEnglish (US)
Pages (from-to)1505-1516
Number of pages12
JournalNucleic acids research
Volume13
Issue number5
DOIs
StatePublished - Mar 11 1985

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Interleukin-2 Receptors
Complementary DNA
Messenger RNA
COS Cells
Amino Acid Sequence
Exons
Nucleotides
Monoclonal Antibodies
T-Lymphocytes
Antigens
Cell Line

ASJC Scopus subject areas

  • Genetics

Cite this

Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence. / Shimuzu, Akira; Kondo, Shigeru; Takeda, Shun Ichi; Yodoi, Junji; Ishida, Norio; Sabe, Hisataka; Osawa, Hisao; Diamantstein, Tibor; Nikaido, Toshio; Honjo, Tasuku.

In: Nucleic acids research, Vol. 13, No. 5, 11.03.1985, p. 1505-1516.

Research output: Contribution to journalArticle

Shimuzu, A, Kondo, S, Takeda, SI, Yodoi, J, Ishida, N, Sabe, H, Osawa, H, Diamantstein, T, Nikaido, T & Honjo, T 1985, 'Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence', Nucleic acids research, vol. 13, no. 5, pp. 1505-1516. https://doi.org/10.1093/nar/13.5.1505
Shimuzu, Akira ; Kondo, Shigeru ; Takeda, Shun Ichi ; Yodoi, Junji ; Ishida, Norio ; Sabe, Hisataka ; Osawa, Hisao ; Diamantstein, Tibor ; Nikaido, Toshio ; Honjo, Tasuku. / Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence. In: Nucleic acids research. 1985 ; Vol. 13, No. 5. pp. 1505-1516.
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AU - Ishida, Norio

AU - Sabe, Hisataka

AU - Osawa, Hisao

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AB - We have cloned cDNA encoding the mouse interleukin-2(IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the antigen recognized by the monoclonal antibody against the murine IL-2 receptor. The cDNA identified 4 species of mRNA (4.5, 3.5, 2.2 and 1.5 kb) of the mouse IL-2 receptor in CTLL cells. Difference in the length of mRNA seems to be ascribed to the variable length of the 3′ untranslated sequence. Total nucleotide sequence (̃1400 bp) of this cDNA was determined and compared with the human receptor. The nucleotide and amino acid sequences of the IL-2 receptor are 70% and 60%, respectively, homologous in average between the two species. The comparison has revealed several conserved regions localized to particular exons such as transmembrane and cytoplasmic portions, suggesting that these regions are important for receptor function and its regulation.

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