Overexpression of regenerating gene Iα appears to reflect aberration of crypt cell compartmentalization in sessile serrated adenoma/polyps of the colon

Kentaro Okamoto, Takahiro Fujimori, Takeshi Yamaguchi, Kazuhito Ichikawa, Shigeki Tomita, Tamotsu Sugai, Johji Imura, Yasuo Ohkura, Takashi Yao, Shigehiko Fujii, Toshihiro Kusaka, Akira Sekikawa, Hirokazu Fukui, Tsutomu Chiba, Hiroyuki Kato, Hiroyuki Mitomi

    Research output: Contribution to journalArticle

    3 Scopus citations

    Abstract

    Background: Colorectal sessile serrated adenoma/polyps (SSA/Ps) are characterized by asymmetrical distribution of Ki67-positive cells, which varies among crypts and involves the crypt length to a variable extent; the pattern has been designated as aberration of crypt cell compartmentalization. The regenerating gene (REG) Iα is a cell growth and/or anti-apoptotic factor and its overexpression might be associated with aberration of crypt cell compartmentalization in SSA/Ps. We investigated REG Iα expression in SSA/Ps in comparison to hyperplastic polyps (HPs).Methods: A total of 64 cases of serrated polyps (≥10 mm in size), including 53 SSA/Ps and 11 HPs, were included in the present study. Immunostaining was performed using a labeled streptavidin-biotin method. REG Iα expression was classified as follows: (i) expression of endocrine cells: grade 0 (a few positive cells) to 3 (marked increase in positive cells); (ii) expression of goblet cells: grade 0 (negative) to 2 (positive for crypts and surface epithelial cells); (iii) staining intensity of goblet cells: grade 0 (negative) to 2 (strong); (iv) staining intensity of crypt (absorptive) cell membranes: grade 0 (negative) to 2 (strong). The presence of aberration of crypt cell compartmentalization was assessed using Ki67 immunostaining.Results: With regard to the REG Iα expression of endocrine cells, 8 out of 11 HPs (73%) were grade 0, whereas 51 of 53 SSA/Ps (96%) were grade 1 or higher (p < 0.001). With regard to the distribution of REG Iα-immunoreactive goblet cells, 10 of 11 HPs (91%) were grade 1, whereas 50 of 53 SSA/Ps (94%) were grade 2 (p < 0.001). A similar trend was found in the staining intensity of goblet cells or crypt cell membranes (p = 0.011). Aberration of crypt cell compartmentalization was more frequently identified in SSA/Ps (72%) than in HPs (18%; p = 0.002). A significant association was observed between REG Iα overexpression and the aberration of crypt cell compartmentalization in serrated polyps (p = 0.037).Conclusions: REG Iα overexpression is a characteristic of SSA/Ps, which appears to reflect aberration of crypt cell compartmentalization.Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7240956081100040.

    Original languageEnglish (US)
    Article number187
    JournalDiagnostic pathology
    Volume8
    Issue number1
    DOIs
    StatePublished - Nov 13 2013

      Fingerprint

    Keywords

    • Colon
    • Crypt cell compartmentalization
    • Hyperplastic polyp
    • REG Iα
    • Sessile serrated adenoma/polyp

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Histology

    Cite this

    Okamoto, K., Fujimori, T., Yamaguchi, T., Ichikawa, K., Tomita, S., Sugai, T., Imura, J., Ohkura, Y., Yao, T., Fujii, S., Kusaka, T., Sekikawa, A., Fukui, H., Chiba, T., Kato, H., & Mitomi, H. (2013). Overexpression of regenerating gene Iα appears to reflect aberration of crypt cell compartmentalization in sessile serrated adenoma/polyps of the colon. Diagnostic pathology, 8(1), [187]. https://doi.org/10.1186/1746-1596-8-187