Abstract
Studying HIV-1 replication in the presence of functionally related proteins from different species has helped define host determinants of HIV-1 infection. Humans and owl monkeys, but not macaques, encode a CD4 receptor that permits entry of transmissible HIV-1 variants due to a single residue difference. However, little is known about whether divergent CCR5 receptor proteins act as determinants of host-range. Here we show that both owl monkey (Aotus vociferans) CD4 and CCR5 receptors are functional for the entry of transmitted HIV-1 when paired with human versions of the other receptor. By contrast, the owl monkey CD4/CCR5 pair is generally a suboptimal receptor combination, although there is virus-specific variation in infection with owl monkey receptors. Introduction of the human residues 15Y and 16T within a sulfation motif into owl monkey CCR5 resulted in a gain of function. These findings suggest there is cross-talk between CD4 and CCR5 involving the sulfation motif.
Original language | English (US) |
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Pages (from-to) | 180-186 |
Number of pages | 7 |
Journal | Virology |
Volume | 512 |
DOIs | |
State | Published - Dec 2017 |
Keywords
- CCR5
- CD4
- Entry
- HIV-1
- Owl monkey
- Receptor
- Species differences
ASJC Scopus subject areas
- Virology