Parallelized microfluidic immuno capture of circulating pancreatic cells for genetic analysis and early detection of pancreatic carcinogenesis

F. L. Thege, S. M. Santana, T. B. Lannin, S. Tsai, T. N. Saha, M. E. Godla, E. D. Pratt, A. D. Rhim, B. J. Kirby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed and optimized a novel microfluidic platform for (he earlv detection of pancreatic carcinogenesis. The capture of circulating pancreatic cells (CPCs) was optimized with parallelized anti-epithelial cell adhesion molecule (EpCAM) and cancer-specific anti-mucin 1 (MUC1) immunocapture. Furthermore, we showed release of intact nuclei from captured cells and genetic analysis of DNA from captured cells. In clinical samples, we found CPCs in 67% of pancreatic ductal adenocarcinoma (PDAC) and 36% precancerous pancreas cyst lesion (PCLs) patients. Our data, together with mounting evidence that CPC dissemination is an early event in pancreatic carcinogenesis [1] indicates the diagnostic potential of our platform.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages20-22
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Circulating pancreatic cells
  • Circulating tumor cells
  • Early detection
  • Immunocapture
  • Mucin expression
  • Pancreatic cancer

ASJC Scopus subject areas

  • Bioengineering

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