Participation of p97Eps8 in Src-mediated Transformation

Tzeng Horng Leu, Hsu Hua Yeh, Ching Chung Huang, Ya Chun Chuang, Shu Li Su, Ming Chei Maa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Histone acetylase and histone deacetylase are two crucial enzymes that determine the structure of chromatin, regulating gene expression. In this study, we observed that trichostatin A (TSA), a specific histone deacetylase inhibitor, could effectively inhibit the growth of v-Src-transformed (IV5) cells and abrogate their ability to form colonies in soft agar. Further analysis demonstrated that, although TSA reduced the expression of Eps8 in a dose- and time-dependent manner, both the protein expression and kinase activity of v-Src remained constant, and the abundance and phosphotyrosine levels of Src substrates, including cortactin, focal adhesion kinase, p130 Cas, paxillin, and Shc, were not altered. Notably, removal of TSA from the medium restored not only the expression of Eps8, but also cellular growth. Northern and reverse transcription-PCR analyses revealed the significant reduction of eps8 transcripts in TSA-treated IV5 cells relative to control cells. When active Src-expressing chicken embryonic cells were forced to overexpress p97Eps8, they became resistant to TSA-mediated anti-proliferation. Furthermore, using small interference RNA of eps8, we demonstrated the requirement for Eps8 in IV5 cell proliferation. Thus, our results highlight a critical role for p97Eps8 in TSA-exerted growth inhibition of v-Src-transformed cells.

Original languageEnglish (US)
Pages (from-to)9875-9881
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number11
DOIs
StatePublished - Mar 12 2004

Fingerprint

trichostatin A
Growth
Cortactin
Paxillin
Histone Acetyltransferases
Focal Adhesion Protein-Tyrosine Kinases
Phosphotyrosine
Histone Deacetylase Inhibitors
Histone Deacetylases
Cell proliferation
Transcription
RNA Interference
Gene expression
Protein Kinases
Reverse Transcription
Agar
Chromatin
Chickens
Phosphotransferases
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Leu, T. H., Yeh, H. H., Huang, C. C., Chuang, Y. C., Su, S. L., & Maa, M. C. (2004). Participation of p97Eps8 in Src-mediated Transformation. Journal of Biological Chemistry, 279(11), 9875-9881. https://doi.org/10.1074/jbc.M309884200

Participation of p97Eps8 in Src-mediated Transformation. / Leu, Tzeng Horng; Yeh, Hsu Hua; Huang, Ching Chung; Chuang, Ya Chun; Su, Shu Li; Maa, Ming Chei.

In: Journal of Biological Chemistry, Vol. 279, No. 11, 12.03.2004, p. 9875-9881.

Research output: Contribution to journalArticle

Leu, TH, Yeh, HH, Huang, CC, Chuang, YC, Su, SL & Maa, MC 2004, 'Participation of p97Eps8 in Src-mediated Transformation', Journal of Biological Chemistry, vol. 279, no. 11, pp. 9875-9881. https://doi.org/10.1074/jbc.M309884200
Leu, Tzeng Horng ; Yeh, Hsu Hua ; Huang, Ching Chung ; Chuang, Ya Chun ; Su, Shu Li ; Maa, Ming Chei. / Participation of p97Eps8 in Src-mediated Transformation. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 11. pp. 9875-9881.
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