PD-L1 expression patterns in tumour cells and their association with CD8 + tumour infiltrating lymphocytes in clear cell renal cell carcinoma

Qian Zhu, Mu Yan Cai, De Sheng Weng, Jing Jing Zhao, Qiu Zhong Pan, Qi Jing Wang, Yan Tang, Jia He, Min Li, Xia Jian-Chuan Xia

Research output: Contribution to journalArticle

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Abstract

Purpose: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC). Materials and Methods: 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8+TILs at mRNA level. The in vitro incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis. Results: Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment in vitro demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8+ T cell infiltration. Conclusion: The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8+ TILs. TC-PD-L1-positive ccRCC patients with abundant CD8+ TILs infiltration confer the high risk of death and disease relapse.

Original languageEnglish (US)
Pages (from-to)1154-1161
Number of pages8
JournalJournal of Cancer
Volume10
Issue number5
DOIs
StatePublished - Jan 1 2019

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Tumor-Infiltrating Lymphocytes
Renal Cell Carcinoma
Neoplasms
Survival Analysis
Interferon-gamma
Multivariate Analysis
T-Lymphocytes
Recurrence
Cell Line
Messenger RNA

Keywords

  • Clear cell renal cell carcinoma
  • Extrinsic expression
  • Intrinsic expression
  • Local microenvironment
  • PD-L1

ASJC Scopus subject areas

  • Oncology

Cite this

PD-L1 expression patterns in tumour cells and their association with CD8 + tumour infiltrating lymphocytes in clear cell renal cell carcinoma . / Zhu, Qian; Cai, Mu Yan; Weng, De Sheng; Zhao, Jing Jing; Pan, Qiu Zhong; Wang, Qi Jing; Tang, Yan; He, Jia; Li, Min; Xia, Xia Jian-Chuan.

In: Journal of Cancer, Vol. 10, No. 5, 01.01.2019, p. 1154-1161.

Research output: Contribution to journalArticle

Zhu, Qian ; Cai, Mu Yan ; Weng, De Sheng ; Zhao, Jing Jing ; Pan, Qiu Zhong ; Wang, Qi Jing ; Tang, Yan ; He, Jia ; Li, Min ; Xia, Xia Jian-Chuan. / PD-L1 expression patterns in tumour cells and their association with CD8 + tumour infiltrating lymphocytes in clear cell renal cell carcinoma In: Journal of Cancer. 2019 ; Vol. 10, No. 5. pp. 1154-1161.
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title = "PD-L1 expression patterns in tumour cells and their association with CD8 + tumour infiltrating lymphocytes in clear cell renal cell carcinoma",
abstract = "Purpose: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC). Materials and Methods: 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8+TILs at mRNA level. The in vitro incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis. Results: Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment in vitro demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8+ T cell infiltration. Conclusion: The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8+ TILs. TC-PD-L1-positive ccRCC patients with abundant CD8+ TILs infiltration confer the high risk of death and disease relapse.",
keywords = "Clear cell renal cell carcinoma, Extrinsic expression, Intrinsic expression, Local microenvironment, PD-L1",
author = "Qian Zhu and Cai, {Mu Yan} and Weng, {De Sheng} and Zhao, {Jing Jing} and Pan, {Qiu Zhong} and Wang, {Qi Jing} and Yan Tang and Jia He and Min Li and Xia, {Xia Jian-Chuan}",
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T1 - PD-L1 expression patterns in tumour cells and their association with CD8 + tumour infiltrating lymphocytes in clear cell renal cell carcinoma

AU - Zhu, Qian

AU - Cai, Mu Yan

AU - Weng, De Sheng

AU - Zhao, Jing Jing

AU - Pan, Qiu Zhong

AU - Wang, Qi Jing

AU - Tang, Yan

AU - He, Jia

AU - Li, Min

AU - Xia, Xia Jian-Chuan

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Purpose: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC). Materials and Methods: 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8+TILs at mRNA level. The in vitro incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis. Results: Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment in vitro demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8+ T cell infiltration. Conclusion: The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8+ TILs. TC-PD-L1-positive ccRCC patients with abundant CD8+ TILs infiltration confer the high risk of death and disease relapse.

AB - Purpose: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC). Materials and Methods: 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8+TILs at mRNA level. The in vitro incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis. Results: Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment in vitro demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8+ T cell infiltration. Conclusion: The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8+ TILs. TC-PD-L1-positive ccRCC patients with abundant CD8+ TILs infiltration confer the high risk of death and disease relapse.

KW - Clear cell renal cell carcinoma

KW - Extrinsic expression

KW - Intrinsic expression

KW - Local microenvironment

KW - PD-L1

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