Photochemical transfection: A technology for efficient light-directed gene delivery

Anders Høgset, Lina Prasmickaite, Marit Helium, Birgit Engesæter, Vibeke M. Olsen, Torunn E. Tjelle, Carl J. Wheeler, Kristian Berg

    Research output: Contribution to journalReview article

    31 Scopus citations

    Abstract

    Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vitro, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.

    Original languageEnglish (US)
    Pages (from-to)97-113
    Number of pages17
    JournalSomatic Cell and Molecular Genetics
    Volume27
    Issue number1-6
    DOIs
    StatePublished - Nov 1 2002

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

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  • Cite this

    Høgset, A., Prasmickaite, L., Helium, M., Engesæter, B., Olsen, V. M., Tjelle, T. E., Wheeler, C. J., & Berg, K. (2002). Photochemical transfection: A technology for efficient light-directed gene delivery. Somatic Cell and Molecular Genetics, 27(1-6), 97-113. https://doi.org/10.1023/A:1022979806314