Photodynamic activity on human erythrocytes by a newly synthesized sensitizer - trithia sapphyrin sulfonate: A preliminary report

D. Parmeswaran, S. Ganesan, K. Thanigaivelu, Simi Pushpan, A. Srinivasan, M. Ravi Kumar, T. K. Chandrasekar

Research output: Contribution to journalConference article

3 Scopus citations

Abstract

A new photosensitizer, Trithia Sapphyrin was synthesized with 3 sulfurs and 2 nitrogen inside the macrocycle. The relative effectiveness is influenced by molecular properties, which control the penetration and distribution of the sensitizer in the system prior to the photon capture. To be precise, the effectiveness of the sensitizer depends on (1) the ability to partition from the bathing medium to a region of the cell membrane where it is exposed to a low polarity environment (2) ability to absorb light in such a low polarity environment (3) its triplet quantum yield in this environment. In view of these factors, the partition coefficient for different concentrations, ranging from 1 to 50 μg/ml of trithia sapphyrin in the Octanol/Saline system was determined. The partition coefficient for 5 μg/ml is observed to be the highest, which indicated the higher partitioning of trithia sapphyrin towards the octanol phase i.e. towards the membrane. The uptake of sensitizer was analyzed in 0.05% hematocrit for different periods of incubation ranging from 5 minutes to 5 hour, in order to find (1) binding of sensitizer to the cell membrane (2) partition of the sensitizer molecule between the aqueous phase and lipid phase and (3) diffusion of the pigment molecule into the intracellular aqueous phase. With these observations, Photohemolysis studies were carried out for different pre and post incubation time as a function of light dose and sensitizer concentration.

Original languageEnglish (US)
Pages (from-to)94-100
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3592
StatePublished - Jan 1 1999
EventProceedings of the 1999 Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII - San Jose, CA, USA
Duration: Jan 23 1999Jan 24 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Photodynamic activity on human erythrocytes by a newly synthesized sensitizer - trithia sapphyrin sulfonate: A preliminary report'. Together they form a unique fingerprint.

  • Cite this

    Parmeswaran, D., Ganesan, S., Thanigaivelu, K., Pushpan, S., Srinivasan, A., Ravi Kumar, M., & Chandrasekar, T. K. (1999). Photodynamic activity on human erythrocytes by a newly synthesized sensitizer - trithia sapphyrin sulfonate: A preliminary report. Proceedings of SPIE - The International Society for Optical Engineering, 3592, 94-100.