@inproceedings{9e07f75a405c4fcabc15749e64dedc53,
title = "Photothermal therapy of acute leukemia cells in the near-infrared region using gold nanorods CD-33 conjugates",
abstract = "In the present work, we demonstrate a potential use of gold nanorods as a contrast agent for selective photothermal therapy of human acute leukemia cells (HL-60) using a near-infrared laser. Gold Nanorods (GNR) are synthesized and conjugated to CD33, a 67 kDa glycoprotein found on the surface of myeloid cells that belongs to the sialoadhesin family of proteins. After pegylation, or conjugation with CD33 antibody, GNR were non-toxic for acute and chronic leukemia cells. We used a Quanta System q-switched titanium sapphire laser emitting at a center wavelength of 755 nm. Each sample was illuminated with 1 laser shot at either high or low fluence. Both laser modes were used in 3 independent cell probes. HL-60 cells were treated for 45 min with GNR conjugated with mAb CD33, or with GNR-Pegylated particles. After laser application, the cells were resuspended and analyzed to cell viability with Trypan blue exclusion assay. GNR-CD33 conjugates significantly increase the percentage of cell death as compared with a control group after laser illumination: a 3 fold increase is observed.",
keywords = "Bubble formation, CD33 antibody, Cell selective targeting, Contrast agents, Gold nanorods conjugates, Human leukemia, Laser nanothermolysis, Optoacoustic",
author = "Anton Liopo and And{\'r} Conjusteau and Marina Konopleva and Michael Andreeff and Alexander Oraevsky",
year = "2011",
doi = "10.1117/12.878802",
language = "English (US)",
isbn = "9780819484345",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Optical Interactions with Tissue and Cells XXII",
note = "Optical Interactions with Tissue and Cells XXII ; Conference date: 24-01-2011 Through 26-01-2011",
}