Photothermal therapy of acute leukemia cells in the near-infrared region using gold nanorods CD-33 conjugates

Anton Liopo, Andŕ Conjusteau, Marina Konopleva, Michael Andreeff, Alexander Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

In the present work, we demonstrate a potential use of gold nanorods as a contrast agent for selective photothermal therapy of human acute leukemia cells (HL-60) using a near-infrared laser. Gold Nanorods (GNR) are synthesized and conjugated to CD33, a 67 kDa glycoprotein found on the surface of myeloid cells that belongs to the sialoadhesin family of proteins. After pegylation, or conjugation with CD33 antibody, GNR were non-toxic for acute and chronic leukemia cells. We used a Quanta System q-switched titanium sapphire laser emitting at a center wavelength of 755 nm. Each sample was illuminated with 1 laser shot at either high or low fluence. Both laser modes were used in 3 independent cell probes. HL-60 cells were treated for 45 min with GNR conjugated with mAb CD33, or with GNR-Pegylated particles. After laser application, the cells were resuspended and analyzed to cell viability with Trypan blue exclusion assay. GNR-CD33 conjugates significantly increase the percentage of cell death as compared with a control group after laser illumination: a 3 fold increase is observed.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXII
DOIs
StatePublished - 2011
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7897
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXII
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Keywords

  • Bubble formation
  • CD33 antibody
  • Cell selective targeting
  • Contrast agents
  • Gold nanorods conjugates
  • Human leukemia
  • Laser nanothermolysis
  • Optoacoustic

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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