Abstract
Serologic and immunochemical techniques were used to characterize plasma membrane antigens of the murine lymphoma 6C3HED. Syngeneic C3H/HeJ anti-6C3HED antisera were prepared and were shown by cytotoxicity, absorption, and immunoprecipitation tests to be specifically reactive with the lymphoma cells. Alloantisera to H-2.23 were found to be unreactive with intact lymphoma cells by cytotoxicity and absorption assays. However, H-2.23 antigens were detectable in tumor cell lysates by inhibition of cytotoxicity and immunoprecipitation. SDS-PAGE electropherograms of immunoprecipitates of radiolabeled tumor cell extracts obtained with anti-6C3HED and anti-H-2.23 antisera were essentially identical; proteins with M(r) of approximately 70,000, 45,000 and 12,000 were apparent in both. An immunoadsorbent of insolubilized anti-6C3HED removed H-2.23 antigens detectable in lymphoma extracts, but had no effect on H-2.23 antigens in normal splenocyte extracts. These data indicate that H-2.23 and TAA are physically associated on 6C3HED cells.
Original language | English (US) |
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Pages (from-to) | 70-74 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 122 |
Issue number | 1 |
State | Published - 1979 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology