TY - JOUR
T1 - Physical mapping and genomic structure of the human TNFR2 gene
AU - Beltinger, Christian P.
AU - White, Peter S.
AU - Maris, John M.
AU - Sulman, Erik P.
AU - Jensen, Sanford J.
AU - LePaslier, Denis
AU - Stallard, Barbara J.
AU - Goeddel, David V.
AU - De Sauvage, Frederic J.
AU - Brodeur, Garrett M.
N1 - Funding Information:
We are grateful to T. Look for the TNFR2 cosmid; to R. Heller for the TNFR2 cDNA clone; to S. Kyemba for technical assistance; to Nic Dracopoli, J. Vance, P. Denton, and the Whitehead Institute/ MIT Genome Center for identi®cation of YAC addresses; to H. Donis-Keller for CEPH pedigree DNAs; and to the Cooperative Human Linkage Center for access to their linkage server. The cDNA from R. Heller was obtained prior to the collaboration with the Genentech investigators (B.J.S., D.V.G., F.J.D.) and was not shared. This work was supported in part by NIH Grant RO1-CA39771 (G.M.B.).
PY - 1996/7/1
Y1 - 1996/7/1
N2 - The tumor necrosis factor receptor 2 (TNFR2) gene localizes to 1p36.2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFR2 is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFR1 in the killing of nonlymphoid cells. Therefore, we undertook an analysis of the genomic structure and precise physical mapping of this gene. The TNFR2 gene is contained on 10 exons that span 26 kb. Most of the functional domains of TNFR2 are encoded by separate exons, and each of the repeats of the extracellular cysteine-rich domain is interrupted by an intron. The genomic structure reveals a close relationship to TNFR1, another member of the TNFR superfamily. Based on electrophoretic analysis of yeast artificial chromosomes, TNFR2 maps within 400 kb of the genetic marker D1S434. In addition, we have identified a new polymorphic dinucleotide repeat within intron 4 of TNFR2. The genetic sequence information and exon-intron boundaries we have determined will facilitate mutational analysis of this gene to determine its potential role in neuroblastoma, as well as in other cancers with characteristic deletions or rearrangements of 1p36.
AB - The tumor necrosis factor receptor 2 (TNFR2) gene localizes to 1p36.2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFR2 is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFR1 in the killing of nonlymphoid cells. Therefore, we undertook an analysis of the genomic structure and precise physical mapping of this gene. The TNFR2 gene is contained on 10 exons that span 26 kb. Most of the functional domains of TNFR2 are encoded by separate exons, and each of the repeats of the extracellular cysteine-rich domain is interrupted by an intron. The genomic structure reveals a close relationship to TNFR1, another member of the TNFR superfamily. Based on electrophoretic analysis of yeast artificial chromosomes, TNFR2 maps within 400 kb of the genetic marker D1S434. In addition, we have identified a new polymorphic dinucleotide repeat within intron 4 of TNFR2. The genetic sequence information and exon-intron boundaries we have determined will facilitate mutational analysis of this gene to determine its potential role in neuroblastoma, as well as in other cancers with characteristic deletions or rearrangements of 1p36.
UR - https://www.scopus.com/pages/publications/0030200734
UR - https://www.scopus.com/pages/publications/0030200734#tab=citedBy
U2 - 10.1006/geno.1996.0327
DO - 10.1006/geno.1996.0327
M3 - Article
C2 - 8661109
AN - SCOPUS:0030200734
SN - 0888-7543
VL - 35
SP - 94
EP - 100
JO - Genomics
JF - Genomics
IS - 1
ER -