Plasma lipid analysis by hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Kazuhiro Sonomura, Shinobu Kudoh, Taka Aki Sato, Fumihiko Matsuda

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    4 Scopus citations


    A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono-hexsosyl ceramide groups with good peak area repeatability (RSD% < 10) and linearity (R2 > 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co-existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage.

    Original languageEnglish (US)
    Pages (from-to)2033-2037
    Number of pages5
    JournalJournal of Separation Science
    Issue number12
    StatePublished - Jun 1 2015



    • Electrospray ionization tandem mass spectrometry
    • Hydrophilic interaction liquid chromatography
    • Liquid chromatography
    • Plasma Lipids

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Filtration and Separation

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