Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius

Silvia Guzmán, Alonso Carmona, Laura Escalante, Iveta Imriskova, Ruth López, Romina Rodríguez-Sanoja, Beatriz Ruiz, Luis Servín-González, Sergio Sánchez, Elizabeth Langley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

SCO2127 and SCO2126 (glkA) are adjacent regions located in Streptomyces coelicolor DNA. glkA encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus Streptomyces. In this work, the glkA and SCO2127 genes from S. coelicolor were used, either individually or together, to transform three mutants of Streptomyces peucetius var. caesius resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the SCO2127 sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative SCO2127 amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in glkA mRNA levels was observed in a CCR-resistant mutant transformed with SCO2127 compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the glkA sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the SCO2127 gene product enhances transcription of both genes, and support the first specific role for this region in Streptomyces species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.

Original languageEnglish (US)
Pages (from-to)1717-1723
Number of pages7
JournalMicrobiology
Volume151
Issue number5
DOIs
StatePublished - May 1 2005

Fingerprint

Catabolite Repression
Streptomyces
Phosphotransferases
Glucose
Genes
Streptomyces coelicolor
Amino Acid Sequence
Reference Values
Plasmids
Messenger RNA

ASJC Scopus subject areas

  • Microbiology

Cite this

Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius. / Guzmán, Silvia; Carmona, Alonso; Escalante, Laura; Imriskova, Iveta; López, Ruth; Rodríguez-Sanoja, Romina; Ruiz, Beatriz; Servín-González, Luis; Sánchez, Sergio; Langley, Elizabeth.

In: Microbiology, Vol. 151, No. 5, 01.05.2005, p. 1717-1723.

Research output: Contribution to journalArticle

Guzmán, S, Carmona, A, Escalante, L, Imriskova, I, López, R, Rodríguez-Sanoja, R, Ruiz, B, Servín-González, L, Sánchez, S & Langley, E 2005, 'Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius', Microbiology, vol. 151, no. 5, pp. 1717-1723. https://doi.org/10.1099/mic.0.27557-0
Guzmán, Silvia ; Carmona, Alonso ; Escalante, Laura ; Imriskova, Iveta ; López, Ruth ; Rodríguez-Sanoja, Romina ; Ruiz, Beatriz ; Servín-González, Luis ; Sánchez, Sergio ; Langley, Elizabeth. / Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius. In: Microbiology. 2005 ; Vol. 151, No. 5. pp. 1717-1723.
@article{45750ce3630840a48795fbc4bc5c1312,
title = "Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius",
abstract = "SCO2127 and SCO2126 (glkA) are adjacent regions located in Streptomyces coelicolor DNA. glkA encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus Streptomyces. In this work, the glkA and SCO2127 genes from S. coelicolor were used, either individually or together, to transform three mutants of Streptomyces peucetius var. caesius resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the SCO2127 sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative SCO2127 amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in glkA mRNA levels was observed in a CCR-resistant mutant transformed with SCO2127 compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the glkA sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the SCO2127 gene product enhances transcription of both genes, and support the first specific role for this region in Streptomyces species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.",
author = "Silvia Guzm{\'a}n and Alonso Carmona and Laura Escalante and Iveta Imriskova and Ruth L{\'o}pez and Romina Rodr{\'i}guez-Sanoja and Beatriz Ruiz and Luis Serv{\'i}n-Gonz{\'a}lez and Sergio S{\'a}nchez and Elizabeth Langley",
year = "2005",
month = "5",
day = "1",
doi = "10.1099/mic.0.27557-0",
language = "English (US)",
volume = "151",
pages = "1717--1723",
journal = "Microbiology (Reading, England)",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "5",

}

TY - JOUR

T1 - Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius

AU - Guzmán, Silvia

AU - Carmona, Alonso

AU - Escalante, Laura

AU - Imriskova, Iveta

AU - López, Ruth

AU - Rodríguez-Sanoja, Romina

AU - Ruiz, Beatriz

AU - Servín-González, Luis

AU - Sánchez, Sergio

AU - Langley, Elizabeth

PY - 2005/5/1

Y1 - 2005/5/1

N2 - SCO2127 and SCO2126 (glkA) are adjacent regions located in Streptomyces coelicolor DNA. glkA encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus Streptomyces. In this work, the glkA and SCO2127 genes from S. coelicolor were used, either individually or together, to transform three mutants of Streptomyces peucetius var. caesius resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the SCO2127 sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative SCO2127 amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in glkA mRNA levels was observed in a CCR-resistant mutant transformed with SCO2127 compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the glkA sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the SCO2127 gene product enhances transcription of both genes, and support the first specific role for this region in Streptomyces species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.

AB - SCO2127 and SCO2126 (glkA) are adjacent regions located in Streptomyces coelicolor DNA. glkA encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus Streptomyces. In this work, the glkA and SCO2127 genes from S. coelicolor were used, either individually or together, to transform three mutants of Streptomyces peucetius var. caesius resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the SCO2127 sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative SCO2127 amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in glkA mRNA levels was observed in a CCR-resistant mutant transformed with SCO2127 compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the glkA sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the SCO2127 gene product enhances transcription of both genes, and support the first specific role for this region in Streptomyces species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.

UR - http://www.scopus.com/inward/record.url?scp=21044444003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21044444003&partnerID=8YFLogxK

U2 - 10.1099/mic.0.27557-0

DO - 10.1099/mic.0.27557-0

M3 - Article

C2 - 15870479

AN - SCOPUS:21044444003

VL - 151

SP - 1717

EP - 1723

JO - Microbiology (Reading, England)

JF - Microbiology (Reading, England)

SN - 1350-0872

IS - 5

ER -