Polyethylenimine and minicircle DNA based gene transfer

Chao Zhang, He Liu, Shijuan Gao, Wenlin Huang, Zongye Wang

    Research output: Contribution to journalArticle

    1 Scopus citations

    Abstract

    Polyethylenimine (PEI) is one of the most characterized non-viral vectors. It can condense DNA in a good manner and achieve high transfection efficiency. Minicircle DNA (mc-DNA) is a novel kind of supercoiled DNA which is devoid of bacterial backbone. mc-DNA is superior to conventional DNA for its higher transfection effciciency and longer time-span. In this study, we combined PEI and mc-DNA in gene delivery system. We investigated the physicochemical and biochemical effects of this non-viral system and further explore its potential in tumor gene therapy. mc-DNA was obtained by recombination of parental plasmid in the presence of L-arabinose, and complexed with PEI. The results of transmission electron microscopy and scanning electron microscopy showed that the particles were spherical and homogeneous. Through gel retardation assay and MTT assay, we found that there were no obvious differences in binding capability of PEI to mc-DNA and plasmid DNA, as well as in cytotoxicity. The results of dynamic light scattering showed that the size of PEI/mc-DNA was about 68 nm, a slight larger than that of PEI/plasmid DNA. Furthermore, the tumor cells transfected with mc-GFP showed higher GFP expression level than that of conventional plasmid. The same results were achieved in the cells treated with tumor-suppressor gene pten, assayed by RT-PCR and Western blot. It indicates that the system of PEI/minicircle DNA is promising in gene transfer.

    Original languageEnglish (US)
    Pages (from-to)772-779
    Number of pages8
    JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
    Volume26
    Issue number6
    StatePublished - Jul 26 2010

    Keywords

    • Gene delivery
    • Gfp
    • Minicircle DNA
    • Nanoparticle
    • Polyethylenimine
    • Pten

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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  • Cite this

    Zhang, C., Liu, H., Gao, S., Huang, W., & Wang, Z. (2010). Polyethylenimine and minicircle DNA based gene transfer. Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, 26(6), 772-779.