Preparation of polymerase chain reaction template DNA from mouse tail tissue

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece from the tip of the tail is removed and processed using hot sodium hydroxide and Tris (HotSHOT).

Original languageEnglish (US)
Pages (from-to)325-326
Number of pages2
JournalCold Spring Harbor protocols
Volume2019
Issue number4
DOIs
StatePublished - Apr 1 2019

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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