Preprocessing and analysis of LC-MS-based proteomic data

Tsung Heng Tsai, Minkun Wang, Habtom W. Ressom

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Scopus citations

Abstract

Liquid chromatography coupled with mass spectrometry (LC-MS) has been widely used for profiling protein expression levels. This chapter is focused on LC-MS data preprocessing, which is a crucial step in the analysis of LC-MS based proteomics. We provide a high-level overview, highlight associated challenges, and present a step-by-step example for analysis of data from LC-MS based untargeted proteomic study. Furthermore, key procedures and relevant issues with the subsequent analysis by multiple reaction monitoring (MRM) are discussed.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-76
Number of pages14
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1362
ISSN (Print)1064-3745

Keywords

  • Data preprocessing
  • Label-free
  • Liquid chromatography-mass spectrometry (LC-MS)
  • Multiple reaction monitoring (MRM)
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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