Probing endogenous fluorophores in cellular cancer models using temporally and spectrally resolved laser induced fluorescence

Paul Urayama; Wei Zhong; Roger D. Sloboda; Konstantin H. Dragnev; Ethan Dmitrovsky; Mary Ann Mycek

Probing endogenous fluorophores in cellular cancer models using temporally and spectrally resolved laser induced fluorescence

Paul Urayama, Wei Zhong, Roger D. Sloboda, Konstantin H. Dragnev, Ethan Dmitrovsky, Mary Ann Mycek

Thoracic Head & Neck Medical Oncology

Research output: Contribution to conference › Paper › peer-review

2 Scopus citations

Abstract

A non-invasive optical method to monitor cellular NADH and probe metabolic activity was illustrated. The autofluorescence intensity differences between cell lines were due to the differing concentrations of protein bound mitochondrial NADH. Fluorescence decays were analyzed by least squares iterative reconvolution to account for instrument response and to extract lifetimes and amplitudes. All data were obtained using an excitation wavelength of 337.1 nm.

Original language	English (US)
Pages	587-588
Number of pages	2
State	Published - 2002
Event	Conference on Lasers and Electro-Optics (CLEO 2002) - Long Beach, CA, United States Duration: May 19 2002 → May 24 2002

Other

Other	Conference on Lasers and Electro-Optics (CLEO 2002)
Country/Territory	United States
City	Long Beach, CA
Period	5/19/02 → 5/24/02

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
Electrical and Electronic Engineering

Cite this

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title = "Probing endogenous fluorophores in cellular cancer models using temporally and spectrally resolved laser induced fluorescence",

abstract = "A non-invasive optical method to monitor cellular NADH and probe metabolic activity was illustrated. The autofluorescence intensity differences between cell lines were due to the differing concentrations of protein bound mitochondrial NADH. Fluorescence decays were analyzed by least squares iterative reconvolution to account for instrument response and to extract lifetimes and amplitudes. All data were obtained using an excitation wavelength of 337.1 nm.",

author = "Paul Urayama and Wei Zhong and Sloboda, {Roger D.} and Dragnev, {Konstantin H.} and Ethan Dmitrovsky and Mycek, {Mary Ann}",

year = "2002",

language = "English (US)",

pages = "587--588",

}

TY - CONF

T1 - Probing endogenous fluorophores in cellular cancer models using temporally and spectrally resolved laser induced fluorescence

AU - Urayama, Paul

AU - Zhong, Wei

AU - Sloboda, Roger D.

AU - Dragnev, Konstantin H.

AU - Dmitrovsky, Ethan

AU - Mycek, Mary Ann

PY - 2002

Y1 - 2002

N2 - A non-invasive optical method to monitor cellular NADH and probe metabolic activity was illustrated. The autofluorescence intensity differences between cell lines were due to the differing concentrations of protein bound mitochondrial NADH. Fluorescence decays were analyzed by least squares iterative reconvolution to account for instrument response and to extract lifetimes and amplitudes. All data were obtained using an excitation wavelength of 337.1 nm.

AB - A non-invasive optical method to monitor cellular NADH and probe metabolic activity was illustrated. The autofluorescence intensity differences between cell lines were due to the differing concentrations of protein bound mitochondrial NADH. Fluorescence decays were analyzed by least squares iterative reconvolution to account for instrument response and to extract lifetimes and amplitudes. All data were obtained using an excitation wavelength of 337.1 nm.

UR - http://www.scopus.com/inward/record.url?scp=0036457339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036457339&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:0036457339

SP - 587

EP - 588

T2 - Conference on Lasers and Electro-Optics (CLEO 2002)

Y2 - 19 May 2002 through 24 May 2002

ER -

Probing endogenous fluorophores in cellular cancer models using temporally and spectrally resolved laser induced fluorescence

Abstract

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ASJC Scopus subject areas

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