Prognostic significance of Flotillin1 expression in clinically N0 tongue squamous cell cancer

Huan Li, Yan Zhang, Shu Wei Chen, Feng Jiao Li, Shi Min Zhuang, Li Ping Wang, Ji Zhang, Ming Song

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose: The present study aimed to investigate the clinical and prognostic significance of Flotillin1 (FLOT1) in clinically N0 tongue squamous cell cancer (cN0 TSCC). Methods: Real-time PCR and Western blotting analyses were carried out to examine FLOT1 expression in four tongue squamous cell cancer cell lines, primary cultured normal tongue epithelial cells, and eight matched pairs of oral tongue cancer samples and adjacent non-cancerous tissue samples from the same patient. Immunohistochemistry was performed to examine FLOT1 protein expression in paraffin-embedded tissues from 181 cN0 TSCC patients. Statistical analyses evaluated the diagnostic value and the associations of FLOT1 expression with clinical parameters. Results: FLOT1 mRNA and protein levels were upregulated in tongue squamous cell cancer cell lines and cancerous tissues compared with that in TEC and adjacent non-cancerous tissue samples. The level of FLOT1 protein was positively correlated with clinical stage (P = 0.001), T classification (P = 0.009), N classification (P = 0.001) and recurrence (P = 0.018). Patients with higher FLOT1 expression had shorter overall survival times. Conclusion: Our results suggest that overexpression of FLOT1 can be found in patients with higher pathological stage, T classification, N classification or recurrence. FLOT1 expression is associated with cN0 TSCC progression and may be valuable for the prognostic evaluation of cN0 TSCC.

Original languageEnglish (US)
Pages (from-to)996-1003
Number of pages8
JournalInternational journal of clinical and experimental pathology
Volume7
Issue number3
StatePublished - Jan 1 2014

Fingerprint

Tongue Neoplasms
Squamous Cell Neoplasms
Recurrence
Cell Line
Proteins
Mouth Neoplasms
Tongue
Paraffin
Real-Time Polymerase Chain Reaction
Western Blotting
Epithelial Cells
Immunohistochemistry
Messenger RNA
Survival

Keywords

  • Clinically N0 tongue squamous cell cancer
  • FLOT1
  • Flotillin1
  • Prognosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Prognostic significance of Flotillin1 expression in clinically N0 tongue squamous cell cancer. / Li, Huan; Zhang, Yan; Chen, Shu Wei; Li, Feng Jiao; Zhuang, Shi Min; Wang, Li Ping; Zhang, Ji; Song, Ming.

In: International journal of clinical and experimental pathology, Vol. 7, No. 3, 01.01.2014, p. 996-1003.

Research output: Contribution to journalArticle

Li, Huan ; Zhang, Yan ; Chen, Shu Wei ; Li, Feng Jiao ; Zhuang, Shi Min ; Wang, Li Ping ; Zhang, Ji ; Song, Ming. / Prognostic significance of Flotillin1 expression in clinically N0 tongue squamous cell cancer. In: International journal of clinical and experimental pathology. 2014 ; Vol. 7, No. 3. pp. 996-1003.
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