Estudio del patrón de metilación génico en el cáncer gástrico en Chile

Translated title of the contribution: Promoter methylation profile in gastric cancer

Juan Carlos Roa S, Leonardo Anabalón R, Ivan Roa, Oscar Tapia E, Angélica Melo, Miguel Villaseca H, Juan Carlos Araya O

    Research output: Contribution to journalArticle

    12 Scopus citations

    Abstract

    Background: Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies. Aim: To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with gastric cancer (GC) and the relationship with its morphological features and biological behavior. Material and methods: Forty-seven fresh-frozen GC samples were selected. The methylation-specific PCR (MSP) test was used to analyze promoter methylation status for genes MLH1, CDKN2A (p16), APC, CDH1 (Cadherin E) and FHIT. Follow-up and complete morphological features were obtained for all cases. Results: We found methylation in at least one of the genes studied in 83% of the cases. The frequencies of promoter hypermethylation of MLH1, CDKN2A, APC, CDH1 and FHIT were 31%, 43%, 46%, 80% y 62%, respectively. We found a relationship between APC methylation and good histological differentiation (p=0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04). We also found a non-significant relationship between CDKN2A methylation and better survival (p=0.07). Conclusions: The high frequency promoter methylation found confirms its importance in gastric carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search for new criteria in clinical and therapeutic decision making.

    Translated title of the contributionPromoter methylation profile in gastric cancer
    Original languageSpanish
    Pages (from-to)874-880
    Number of pages7
    JournalRevista Medica de Chile
    Volume133
    Issue number8
    StatePublished - Nov 21 2005

    Keywords

    • DNA methylation
    • Promoter regions (Genetics)
    • Stomach neoplasms

    ASJC Scopus subject areas

    • Medicine(all)

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  • Cite this

    Roa S, J. C., Anabalón R, L., Roa, I., Tapia E, O., Melo, A., Villaseca H, M., & Araya O, J. C. (2005). Estudio del patrón de metilación génico en el cáncer gástrico en Chile. Revista Medica de Chile, 133(8), 874-880.