Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling

Fadi Abdi; Bulent Ozpolat; Sheng Gu; E. M. Bradbury; Gabriel Lopez-Berestein; Xian Chen

Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling

Fadi Abdi, Bulent Ozpolat, Sheng Gu, E. M. Bradbury, Gabriel Lopez-Berestein, Xian Chen

Experimental Therapeutics

Research output: Contribution to conference › Paper › peer-review

Abstract

The proteomics analysis of All-trans-Retinoic Acid (ATRA) treated and untreated Acute Promyelocytic Leukemia (APL) cells to identify differentially expresses proteins was investigated. A site-specific tagging method with LC/MS/MS and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was employed to study differential protein expression in APL cell line in an attempt to identify molecular markers carrying a clinical relevance. The Protein Prospector database was used to perform Protein identification. The proteins with functions related to cell proliferations, cell cycle apoptosis, tumor suppression, and several proteins with unknown functions were revealed by LC/MS/MS and MALDI-TOF-MS analyses.

Original language	English (US)
Pages	293-294
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Abdi, F., Ozpolat, B., Gu, S., Bradbury, E. M., Lopez-Berestein, G., & Chen, X. (2002). Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling. 293-294. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling. / Abdi, Fadi; Ozpolat, Bulent; Gu, Sheng et al.
2002. 293-294 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Abdi, F, Ozpolat, B, Gu, S, Bradbury, EM, Lopez-Berestein, G & Chen, X 2002, 'Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 293-294.

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abstract = "The proteomics analysis of All-trans-Retinoic Acid (ATRA) treated and untreated Acute Promyelocytic Leukemia (APL) cells to identify differentially expresses proteins was investigated. A site-specific tagging method with LC/MS/MS and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was employed to study differential protein expression in APL cell line in an attempt to identify molecular markers carrying a clinical relevance. The Protein Prospector database was used to perform Protein identification. The proteins with functions related to cell proliferations, cell cycle apoptosis, tumor suppression, and several proteins with unknown functions were revealed by LC/MS/MS and MALDI-TOF-MS analyses.",

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AU - Lopez-Berestein, Gabriel

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Proteoimic analysis of all-trans-retinoic acid treated acute promyelocytic leukemia cells using mass spectrometry and amino acid specific stable isotope labeling

Abstract

Conference

ASJC Scopus subject areas

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