Proteome profiling uncovers an autoimmune response signature that reflects ovarian cancer pathogenesis

Makoto Kobayashi; Hiroyuki Katayama; Ehsan Irajizad; Jody V. Vykoukal; Johannes F. Fahrmann; Deepali L. Kundnani; Chuan Yih Yu; Yining Cai; Fu Chung Hsiao; Wei Lei Yang; Zhen Lu; Joseph Celestino; James P. Long; Kim Ann Do; Karen H. Lu; Jon J. Ladd; Nicole Urban; Robert C. Bast; Samir M. Hanash

doi:10.3390/cancers12020485

Proteome profiling uncovers an autoimmune response signature that reflects ovarian cancer pathogenesis

Makoto Kobayashi, Hiroyuki Katayama, Ehsan Irajizad, Jody V. Vykoukal, Johannes F. Fahrmann, Deepali L. Kundnani, Chuan Yih Yu, Yining Cai, Fu Chung Hsiao, Wei Lei Yang, Zhen Lu, Joseph Celestino, James P. Long, Kim Ann Do, Karen H. Lu, Jon J. Ladd, Nicole Urban, Robert C. Bast, Samir M. Hanash

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Harnessing the immune response to tumor antigens in the form of autoantibodies, which occurs early during tumor development, has relevance to the detection of cancer at early stages. We conducted an initial screen of antigens associated with an autoantibody response in serous ovarian cancer using recombinant protein arrays. The top 25 recombinants that exhibited increased reactivity with cases compared to controls revealed TP53 and MYC, which are ovarian cancer driver genes, as major network nodes. A mass spectrometry based independent analysis of circulating immunoglobulin (Ig)-bound proteins in ovarian cancer and of ovarian cancer cell surface MHC-II bound peptides also revealed a TP53–MYC related network of antigens. Our findings support the occurrence of a humoral immune response to antigens linked to ovarian cancer driver genes that may have utility for early detection applications.

Original language	English (US)
Article number	485
Journal	Cancers
Volume	12
Issue number	2
DOIs	https://doi.org/10.3390/cancers12020485
State	Published - Feb 2020

Keywords

Antibody complexes
Antigen
Autoantibody signature
MYC network
Ovarian cancer
TP53

ASJC Scopus subject areas

Oncology
Cancer Research

MD Anderson CCSG core facilities

Biostatistics Resource Group

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10.3390/cancers12020485

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Kobayashi, M., Katayama, H., Irajizad, E., Vykoukal, J. V., Fahrmann, J. F., Kundnani, D. L., Yu, C. Y., Cai, Y., Hsiao, F. C., Yang, W. L., Lu, Z., Celestino, J., Long, J. P., Do, K. A., Lu, K. H., Ladd, J. J., Urban, N., Bast, R. C., & Hanash, S. M. (2020). Proteome profiling uncovers an autoimmune response signature that reflects ovarian cancer pathogenesis. Cancers, 12(2), Article 485. https://doi.org/10.3390/cancers12020485

Kobayashi, M, Katayama, H , Irajizad, E , Vykoukal, JV , Fahrmann, JF, Kundnani, DL, Yu, CY, Cai, Y , Hsiao, FC, Yang, WL, Lu, Z, Celestino, J, Long, JP , Do, KA , Lu, KH, Ladd, JJ, Urban, N, Bast, RC & Hanash, SM 2020, 'Proteome profiling uncovers an autoimmune response signature that reflects ovarian cancer pathogenesis', Cancers, vol. 12, no. 2, 485. https://doi.org/10.3390/cancers12020485

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abstract = "Harnessing the immune response to tumor antigens in the form of autoantibodies, which occurs early during tumor development, has relevance to the detection of cancer at early stages. We conducted an initial screen of antigens associated with an autoantibody response in serous ovarian cancer using recombinant protein arrays. The top 25 recombinants that exhibited increased reactivity with cases compared to controls revealed TP53 and MYC, which are ovarian cancer driver genes, as major network nodes. A mass spectrometry based independent analysis of circulating immunoglobulin (Ig)-bound proteins in ovarian cancer and of ovarian cancer cell surface MHC-II bound peptides also revealed a TP53–MYC related network of antigens. Our findings support the occurrence of a humoral immune response to antigens linked to ovarian cancer driver genes that may have utility for early detection applications.",

keywords = "Antibody complexes, Antigen, Autoantibody signature, MYC network, Ovarian cancer, TP53",

author = "Makoto Kobayashi and Hiroyuki Katayama and Ehsan Irajizad and Vykoukal, {Jody V.} and Fahrmann, {Johannes F.} and Kundnani, {Deepali L.} and Yu, {Chuan Yih} and Yining Cai and Hsiao, {Fu Chung} and Yang, {Wei Lei} and Zhen Lu and Joseph Celestino and Long, {James P.} and Do, {Kim Ann} and Lu, {Karen H.} and Ladd, {Jon J.} and Nicole Urban and Bast, {Robert C.} and Hanash, {Samir M.}",

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language = "English (US)",

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AU - Fahrmann, Johannes F.

AU - Kundnani, Deepali L.

AU - Yu, Chuan Yih

AU - Cai, Yining

AU - Hsiao, Fu Chung

AU - Yang, Wei Lei

AU - Lu, Zhen

AU - Celestino, Joseph

AU - Long, James P.

AU - Do, Kim Ann

AU - Lu, Karen H.

AU - Ladd, Jon J.

AU - Urban, Nicole

AU - Bast, Robert C.

AU - Hanash, Samir M.

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AB - Harnessing the immune response to tumor antigens in the form of autoantibodies, which occurs early during tumor development, has relevance to the detection of cancer at early stages. We conducted an initial screen of antigens associated with an autoantibody response in serous ovarian cancer using recombinant protein arrays. The top 25 recombinants that exhibited increased reactivity with cases compared to controls revealed TP53 and MYC, which are ovarian cancer driver genes, as major network nodes. A mass spectrometry based independent analysis of circulating immunoglobulin (Ig)-bound proteins in ovarian cancer and of ovarian cancer cell surface MHC-II bound peptides also revealed a TP53–MYC related network of antigens. Our findings support the occurrence of a humoral immune response to antigens linked to ovarian cancer driver genes that may have utility for early detection applications.

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Proteome profiling uncovers an autoimmune response signature that reflects ovarian cancer pathogenesis

Abstract

Keywords

ASJC Scopus subject areas

MD Anderson CCSG core facilities

Access to Document

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