Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells

Keyphrases

• Mammalian Cells 100%
• Mass Spectrometry 100%
• Protein-protein Interaction Network 100%
• Tandem Affinity Purification 100%
• Protein Interactors 100%
• Computational Model 50%
• Functional Studies 50%
• Interacting Proteins 50%
• Bona Fide 50%
• Network Applications 50%
• Modified Protocol 50%
• Affinity Purification-mass Spectrometry (AP-MS) 50%
• Protein Purification 50%
• Streptavidin-binding Peptide 50%
• Tandem Mass Tag 50%
• Two-step Purification 50%
• Tagging System 50%

Biochemistry, Genetics and Molecular Biology

• Mass Spectrometry 100%
• Protein-Protein Interaction Networks 100%
• Tandem Affinity Purification 100%
• Copurification 50%
• Streptavidin 50%
• Protein Purification 50%