Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish

Research output: Contribution to journalArticlepeer-review

Abstract

Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species. For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).

Original languageEnglish (US)
Article number100963
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021

Keywords

  • Cell Biology
  • Microbiology
  • Model Organisms
  • Molecular Biology

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine

MD Anderson CCSG core facilities

  • Research Animal Support Facility

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