Abstract
Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species. For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).
Original language | English (US) |
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Article number | 100963 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 4 |
DOIs | |
State | Published - Dec 17 2021 |
Keywords
- Cell Biology
- Microbiology
- Model Organisms
- Molecular Biology
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine
MD Anderson CCSG core facilities
- Research Animal Support Facility