@article{38b52a2f059c48dd982a304eea11962b,
title = "Protocol for quantitative analysis of pulsatile contractions and cell extrusion in epithelial tissues of larval zebrafish",
abstract = "Cell elimination by extrusion is important for epithelial homeostasis, but knowing when and where cells will extrude has made in vivo studies difficult. Here, we describe a step-by-step protocol for inducing cell extrusion from the larval zebrafish epidermis. We detail how to capture the dynamics of extrusion via time-lapse imaging and describe how existing protocols can be implemented for the analysis of cell shape changes preceding extrusion events and derivation of mechanical measurements associated with these shape changes. For complete details on the use and execution of this protocol, please refer to Atieh et al. (2021).",
keywords = "cell biology, developmental biology, microscopy, model organisms",
author = "Youmna Atieh and Ruiz, {Oscar E.} and Eisenhoffer, {George T.}",
note = "Funding Information: This work was supported by the Cancer Prevention Research Institute of Texas , RR140077 , the National Institute of General Medical Sciences , National Institutes of Health , R01GM124043 , and the Mark and Linda Quick Basic Science Award to G.T.E. Y. A. is an Odyssey fellow and was supported by the Odyssey program and the Houston Endowment, Inc. at the University of Texas MD Anderson Cancer Center . Zebrafish schematic in the graphical abstract reprinted with permission from ( Brock et al., 2019 ). Funding Information: This work was supported by the Cancer Prevention Research Institute of Texas, RR140077, the National Institute of General Medical Sciences, National Institutes of Health, R01GM124043, and the Mark and Linda Quick Basic Science Award to G.T.E. Y. A. is an Odyssey fellow and was supported by the Odyssey program and the Houston Endowment, Inc. at the University of Texas MD Anderson Cancer Center. Zebrafish schematic in the graphical abstract reprinted with permission from (Brock et al. 2019). Y.A, O.E.R. and G.T.E conceived the method and protocol. Y.A. performed all the experiments and subsequent analysis. Y.A. and G.T.E. wrote the manuscript. The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100600",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}