Quantitative determination of surface concentration of human apolipoprotein H with capillary electrophoresis

Shao Xiong Wang, Yutong Sun, Qin Hua Ru, Guo An Luo, Sen Fang Sui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The phospholipid monolayer at an air/water interface is widely used to mimic the biological membrane. The dynamic process of the protein or peptide interacting with lipid molecules can be reflected in the change in surface pressure of the monolayer. But the conventional method used to measure the surface pressure change gives results that cannot easily be correlated with the contribution of a single protein molecule. Previously, measuring the surface concentration of the protein molecules at the air/water interface has required the protein to be labeled with radioactivity or fluorescence. Here, a new method using capillary electrophoresis is introduced to measure the surface concentration of the protein. The results show at least two advantages of the new method: The numerical results of protein concentration can be obtained in a more precise and rapid way; and there is no need to label the protein sample or to build a special monolayer setup.

Original languageEnglish (US)
Pages (from-to)437-440
Number of pages4
JournalIUBMB Life
Volume49
Issue number5
StatePublished - Jul 17 2000

Fingerprint

beta 2-Glycoprotein I
Capillary electrophoresis
Capillary Electrophoresis
Monolayers
Proteins
Molecules
Membrane Proteins
Air
Biological membranes
Pressure
Water
Radioactivity
Labels
Phospholipids
Fluorescence
Lipids
Peptides
Membranes

Keywords

  • Apolipoprotein H
  • Capillary electrophoresis
  • Membrane insertion
  • Phospholipid monolayer
  • β-glycoprotein-I

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Quantitative determination of surface concentration of human apolipoprotein H with capillary electrophoresis. / Wang, Shao Xiong; Sun, Yutong; Ru, Qin Hua; Luo, Guo An; Sui, Sen Fang.

In: IUBMB Life, Vol. 49, No. 5, 17.07.2000, p. 437-440.

Research output: Contribution to journalArticle

Wang, Shao Xiong ; Sun, Yutong ; Ru, Qin Hua ; Luo, Guo An ; Sui, Sen Fang. / Quantitative determination of surface concentration of human apolipoprotein H with capillary electrophoresis. In: IUBMB Life. 2000 ; Vol. 49, No. 5. pp. 437-440.
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