Radioimmunoassay for the detection and quantitation of bruceantin

Kei Lai L. Fong; Dah Hsi W. Ho; Carol J.K. Carter; Nita S. Brown; Robert S. Benjamin; Emil J. Freireich; Gerald P. Bodey

doi:10.1016/0003-2697(80)90458-3

Radioimmunoassay for the detection and quantitation of bruceantin

Kei Lai L. Fong
, Dah Hsi W. Ho
, Carol J.K. Carter
, Nita S. Brown
, Robert S. Benjamin
, Emil J. Freireich
, Gerald P. Bodey

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A radioimmunoassay for a new anticancer drug, bruceantin, has been developed using [³H]acetylbruceantin and antibody induced by immunizing rabbits with succinylbruceantin-bovine serum albumin conjugates. [³H]Acetylbruceantin was synthesized by reacting bruceantin with [³H]acetyl anhydride. The assay is simple and reproducible. The standard curve was linear on a logit-log plot, and the lower limit of sensitivity of the assay was 1 ng/ml. Using this assay, drug levels were easily determined in tissues of experimental animals following bruceantin administration. The assay procedure does not require sample extraction for plasma, urine, and bile. Bruceantin in other tissues can be extracted quantitatively with ethanol before being measured by the radioimmunoassay.

Original language	English (US)
Pages (from-to)	281-286
Number of pages	6
Journal	Analytical Biochemistry
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90458-3
State	Published - Jun 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{bcc6e86520e7498888473ed24e1f37aa,

title = "Radioimmunoassay for the detection and quantitation of bruceantin",

abstract = "A radioimmunoassay for a new anticancer drug, bruceantin, has been developed using [3H]acetylbruceantin and antibody induced by immunizing rabbits with succinylbruceantin-bovine serum albumin conjugates. [3H]Acetylbruceantin was synthesized by reacting bruceantin with [3H]acetyl anhydride. The assay is simple and reproducible. The standard curve was linear on a logit-log plot, and the lower limit of sensitivity of the assay was 1 ng/ml. Using this assay, drug levels were easily determined in tissues of experimental animals following bruceantin administration. The assay procedure does not require sample extraction for plasma, urine, and bile. Bruceantin in other tissues can be extracted quantitatively with ethanol before being measured by the radioimmunoassay.",

author = "Fong, \{Kei Lai L.\} and Ho, \{Dah Hsi W.\} and Carter, \{Carol J.K.\} and Brown, \{Nita S.\} and Benjamin, \{Robert S.\} and Freireich, \{Emil J.\} and Bodey, \{Gerald P.\}",

note = "Funding Information: I Supported by Grant CA 05831 and Contract CM 87233, Division of Cancer Treatment, National Cancer Treatment, National Institutes of Health, Department of Health, Education and Welfare. 2 To whom reprint requests should be addressed.",

year = "1980",

month = jun,

doi = "10.1016/0003-2697(80)90458-3",

language = "English (US)",

volume = "105",

pages = "281--286",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Radioimmunoassay for the detection and quantitation of bruceantin

AU - Fong, Kei Lai L.

AU - Ho, Dah Hsi W.

AU - Carter, Carol J.K.

AU - Brown, Nita S.

AU - Benjamin, Robert S.

AU - Freireich, Emil J.

AU - Bodey, Gerald P.

N1 - Funding Information: I Supported by Grant CA 05831 and Contract CM 87233, Division of Cancer Treatment, National Cancer Treatment, National Institutes of Health, Department of Health, Education and Welfare. 2 To whom reprint requests should be addressed.

PY - 1980/6

Y1 - 1980/6

N2 - A radioimmunoassay for a new anticancer drug, bruceantin, has been developed using [3H]acetylbruceantin and antibody induced by immunizing rabbits with succinylbruceantin-bovine serum albumin conjugates. [3H]Acetylbruceantin was synthesized by reacting bruceantin with [3H]acetyl anhydride. The assay is simple and reproducible. The standard curve was linear on a logit-log plot, and the lower limit of sensitivity of the assay was 1 ng/ml. Using this assay, drug levels were easily determined in tissues of experimental animals following bruceantin administration. The assay procedure does not require sample extraction for plasma, urine, and bile. Bruceantin in other tissues can be extracted quantitatively with ethanol before being measured by the radioimmunoassay.

AB - A radioimmunoassay for a new anticancer drug, bruceantin, has been developed using [3H]acetylbruceantin and antibody induced by immunizing rabbits with succinylbruceantin-bovine serum albumin conjugates. [3H]Acetylbruceantin was synthesized by reacting bruceantin with [3H]acetyl anhydride. The assay is simple and reproducible. The standard curve was linear on a logit-log plot, and the lower limit of sensitivity of the assay was 1 ng/ml. Using this assay, drug levels were easily determined in tissues of experimental animals following bruceantin administration. The assay procedure does not require sample extraction for plasma, urine, and bile. Bruceantin in other tissues can be extracted quantitatively with ethanol before being measured by the radioimmunoassay.

UR - https://www.scopus.com/pages/publications/0019202241

UR - https://www.scopus.com/pages/publications/0019202241#tab=citedBy

U2 - 10.1016/0003-2697(80)90458-3

DO - 10.1016/0003-2697(80)90458-3

M3 - Article

C2 - 7457834

AN - SCOPUS:0019202241

SN - 0003-2697

VL - 105

SP - 281

EP - 286

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Radioimmunoassay for the detection and quantitation of bruceantin

Abstract

ASJC Scopus subject areas

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