@inbook{60e14559ebbd40d2b2c5d4ca426402ee,
title = "Radiolabeling and imaging of adoptively transferred immune cells by positron emission tomography",
abstract = "Positron emission tomography (PET) using 89Zr is a clinically relevant imaging modality that enables long–term monitoring of adoptively transferred immune cells. This article describes a two-step radiometal labeling procedure utilizing the bifunctional siderophore p-isothiocyanatobenzyl-desferrioxamine (DFO-Bz-NCS) that chelates 89Zr with high affinity and binds covalently to primary amines of cell-surface proteins via its isothiocyanate moiety. Cells labeled with 89Zr-DFO-Bz-NCS remain viable and retain the radiolabel, enabling repetitive PET imaging of adoptively transferred immune cells with high sensitivity and specificity for up to 2 weeks.",
keywords = "Zr, Imaging, Immune cells, p-Isothiocyanatobenzyl-desferrioxamine, PET, Positron emission tomography, Zirconium",
author = "Najjar, {Amer M.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0203-4_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "267--272",
booktitle = "Methods in Molecular Biology",
}