TY - JOUR
T1 - Radium-223 treatment increases immune checkpoint expression in extracellular vesicles from the metastatic prostate cancer bone microenvironment
AU - Vardaki, Ioulia
AU - Corn, Paul
AU - Gentile, Emanuela
AU - Song, Jian H.
AU - Madan, Namrata
AU - Hoang, Anh
AU - Parikh, Nila
AU - Guerra, Leah
AU - Lee, Yu Chen
AU - Lin, Song Chang
AU - Yu, Guoyu
AU - Santos, Elmer
AU - Melancon, Marites P.
AU - Troncoso, Patricia
AU - Navone, Nora
AU - Gallick, Gary E.
AU - Efstathiou, Eleni
AU - Subudhi, Sumit K.
AU - Lin, Sue Hwa
AU - Logothetis, Christopher J.
AU - Panaretakis, Theocharis
N1 - Funding Information:
We acknowledge Bayer for providing Radium-223 for the clinical and preclinical studies. This work was supported by grants from the NIH (CA174798, 5P50 CA140388, P30 CA16672), the Prostate Cancer Foundation, Cancer Prevention and Research Institute of Texas (CPRIT RP150179, RP190252), as well as funds from The University of Texas MD Anderson Moon Shot Program, the Swedish Cancer Foundation (Cancerfonden), and the Radiumhemmets Research Foundation (Radiumhemmets forskning fonder).
Publisher Copyright:
© 2021 American Association for Cancer Research.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Purpose: Radium-223 prolongs survival in a fraction of men with bone metastatic prostate cancer (PCa). However, there are no markers for monitoring response and resistance to Radium-223 treatment. Exosomes are mediators of intercellular communication and may reflect response of the bone microenvironment to Radium-223 treatment. We performed molecular profiling of exosomes and compared the molecular profile in patients with favorable and unfavorable overall survival. Experimental Design: We performed exosomal transcriptome analysis in plasma derived from our preclinical models (MDA-PCa 118b tumors, TRAMP-C2/BMP4 PCa) and from the plasma of 25 patients (paired baseline and end of treatment) treated with Radium-223. All samples were run in duplicate, and array data analyzed with fold changes þ2 to -2 and P < 0.05. Results: We utilized the preclinical models to establish that genes derived from the tumor and the tumor-associated bone microenvironment (bTME) are differentially enriched in plasma exosomes upon Radium-223 treatment. The mouse transcriptome analysis revealed changes in bone-related and DNA damage repair-related pathways. Similar findings were observed in plasma-derived exosomes from patients treated with Radium-223 detected changes. In addition, exosomal transcripts detected immune-suppressors (e.g., PD-L1) that were associated with shorter survival to Radium-223. Treatment of the Myc-CaP mouse model with a combination of Radium-223 and immune checkpoint therapy (ICT) resulted in greater efficacy than monotherapy. Conclusions: These clinical and coclinical analyses showed that RNA profiling of plasma exosomes may be used for monitoring the bTME in response to treatment and that ICT may be used to increase the efficacy of Radium-223.
AB - Purpose: Radium-223 prolongs survival in a fraction of men with bone metastatic prostate cancer (PCa). However, there are no markers for monitoring response and resistance to Radium-223 treatment. Exosomes are mediators of intercellular communication and may reflect response of the bone microenvironment to Radium-223 treatment. We performed molecular profiling of exosomes and compared the molecular profile in patients with favorable and unfavorable overall survival. Experimental Design: We performed exosomal transcriptome analysis in plasma derived from our preclinical models (MDA-PCa 118b tumors, TRAMP-C2/BMP4 PCa) and from the plasma of 25 patients (paired baseline and end of treatment) treated with Radium-223. All samples were run in duplicate, and array data analyzed with fold changes þ2 to -2 and P < 0.05. Results: We utilized the preclinical models to establish that genes derived from the tumor and the tumor-associated bone microenvironment (bTME) are differentially enriched in plasma exosomes upon Radium-223 treatment. The mouse transcriptome analysis revealed changes in bone-related and DNA damage repair-related pathways. Similar findings were observed in plasma-derived exosomes from patients treated with Radium-223 detected changes. In addition, exosomal transcripts detected immune-suppressors (e.g., PD-L1) that were associated with shorter survival to Radium-223. Treatment of the Myc-CaP mouse model with a combination of Radium-223 and immune checkpoint therapy (ICT) resulted in greater efficacy than monotherapy. Conclusions: These clinical and coclinical analyses showed that RNA profiling of plasma exosomes may be used for monitoring the bTME in response to treatment and that ICT may be used to increase the efficacy of Radium-223.
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U2 - 10.1158/1078-0432.CCR-20-4790
DO - 10.1158/1078-0432.CCR-20-4790
M3 - Article
C2 - 33753455
AN - SCOPUS:85107017436
SN - 1078-0432
VL - 27
SP - 3253
EP - 3264
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 11
ER -