TY - JOUR
T1 - Raman imaging in cell membranes, lipid-rich organelles, and lipid bilayers
AU - Syed, Aleem
AU - Smith, Emily A.
N1 - Publisher Copyright:
© 2017 by Annual Reviews.
PY - 2017/6/12
Y1 - 2017/6/12
N2 - Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use in some cellular applications. However, developments in coherent Raman imaging, surface-enhanced Raman imaging, and tip-enhanced Raman imaging have enabled video-rate imaging, excellent detection limits, and nanometer spatial resolution, respectively. After a brief introduction to these commonly used Raman imaging techniques for cell membrane studies, this review discusses selected applications of these modalities for chemical imaging of membrane proteins and lipids. Finally, recent developments in chemical tags for Raman imaging and their applications in the analysis of selected cell membrane components are summarized. Ongoing developments toward improving the temporal and spatial resolution of Raman imaging and small-molecule tags with strong Raman scattering cross sections continue to expand the utility of Raman imaging for diverse cell membrane studies.
AB - Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use in some cellular applications. However, developments in coherent Raman imaging, surface-enhanced Raman imaging, and tip-enhanced Raman imaging have enabled video-rate imaging, excellent detection limits, and nanometer spatial resolution, respectively. After a brief introduction to these commonly used Raman imaging techniques for cell membrane studies, this review discusses selected applications of these modalities for chemical imaging of membrane proteins and lipids. Finally, recent developments in chemical tags for Raman imaging and their applications in the analysis of selected cell membrane components are summarized. Ongoing developments toward improving the temporal and spatial resolution of Raman imaging and small-molecule tags with strong Raman scattering cross sections continue to expand the utility of Raman imaging for diverse cell membrane studies.
KW - Alkyne tags
KW - Coherent anti-Stokes Raman scattering
KW - Deuterium labeling
KW - Stimulated Raman scattering
KW - Surface-enhanced Raman scattering
KW - Tip-enhanced Raman scattering
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U2 - 10.1146/annurev-anchem-061516-045317
DO - 10.1146/annurev-anchem-061516-045317
M3 - Review article
C2 - 28301746
AN - SCOPUS:85020696315
SN - 1936-1327
VL - 10
SP - 271
EP - 291
JO - Annual Review of Analytical Chemistry
JF - Annual Review of Analytical Chemistry
ER -